Involvement of reactive oxygen species in the preservation injury to cultured liver endothelial cells

被引:39
作者
Rauen, U
Elling, B
Gizewski, ER
Korth, HG
Sustmann, R
deGroot, H
机构
[1] UNIV ESSEN GESAMTHSCH KLINIKUM, INST PHYSIOL CHEM, D-45122 ESSEN, GERMANY
[2] UNIV ESSEN GESAMTHSCH, INST ORGAN CHEM, D-45117 ESSEN, GERMANY
关键词
reactive oxygen species; hydroxyl radical; preservation; University of Wisconsin solution; hypothermia; endothelial cells; free radical;
D O I
10.1016/S0891-5849(96)00273-0
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We have previously demonstrated an energy-dependent injury to cultured liver endothelial cells during cold incubation in University of Wisconsin (UW) solution. In the present study, we report experimental evidence for the involvement of reactive oxygen species in this injury: LDH release during 48 h of cold incubation in UW solution was decreased from 40-55% under aerobic conditions to less than 20% under hypoxic conditions or by the presence of KCN (1 mM). Similar protection was achieved by the addition of the spin trap 5,5-dimethyl-1-pyrroline N-oxide, the hydroxyl radical scavenger dimethyl sulfoxide, or the flavonoid silibinin to UW solution under aerobic conditions. Preincubating the cells with the iron chelator deferoxamine even decreased the injury to less than 5%. The residual injury (as observed after longer incubation times) under hypoxic conditions or in cells preincubated with deferoxamine was no longer energy dependent. The amount of thiobarbituric acid-reactive substances markedly increased during cold incubation of the cells in UW solution. This increase was not observed in UW solution to which KCN had been added, i.e., under the conditions of energy depletion. These results suggest that an iron-dependent generation of reactive oxygen species with subsequent lipid peroxidation is involved in the pathogenesis of the injury to cultured liver endothelial cells in cold UW solution. Copyright (C) 1996 Elsevier Science Inc.
引用
收藏
页码:17 / 24
页数:8
相关论文
共 36 条
[1]  
Armitage P., 1971, STAT METHODS MED RES
[2]  
BABBS CF, 1990, METHOD ENZYMOL, V186, P137
[3]   PRINCIPLES OF SOLID-ORGAN PRESERVATION BY COLD-STORAGE [J].
BELZER, FO ;
SOUTHARD, JH .
TRANSPLANTATION, 1988, 45 (04) :673-676
[4]  
BERGMEYER HU, 1974, METHODEN ENZYMATISCH, P607
[5]   LIVER PRESERVATION - THE PAST AND THE FUTURE [J].
BLANKENSTEIJN, JD ;
TERPSTRA, OT .
HEPATOLOGY, 1991, 13 (06) :1235-1250
[6]   RESISTANCE OF ENDOTHELIAL-CELLS TO ANOXIA-REOXYGENATION IN ISOLATED GUINEA-PIG HEARTS [J].
BUDERUS, S ;
SIEGMUND, B ;
SPAHR, R ;
KRUTZFELDT, A ;
PIPER, HM .
AMERICAN JOURNAL OF PHYSIOLOGY, 1989, 257 (02) :H488-H493
[7]   PRESERVATION AND REPERFUSION INJURIES IN LIVER ALLOGRAFTS - AN OVERVIEW AND SYNTHESIS OF CURRENT STUDIES [J].
CLAVIEN, PA ;
HARVEY, PRC ;
STRASBERG, SM .
TRANSPLANTATION, 1992, 53 (05) :957-978
[8]  
CURRIN RT, 1990, TRANSPLANTATION, V50, P1076
[9]   MITOCHONDRIA AS A SOURCE OF REACTIVE OXYGEN SPECIES DURING REDUCTIVE STRESS IN RAT HEPATOCYTES [J].
DAWSON, TL ;
GORES, GJ ;
NIEMINEN, AL ;
HERMAN, B ;
LEMASTERS, JJ .
AMERICAN JOURNAL OF PHYSIOLOGY, 1993, 264 (04) :C961-C967
[10]  
DEGROOT H, 1994, HEPATO-GASTROENTEROL, V41, P328