Dengue Reporter Virus Particles for Measuring Neutralizing Antibodies against Each of the Four Dengue Serotypes

被引：54

作者：

Mattia, Kimberly ^{[1
]}

Puffer, Bridget A. ^{[1
]}

Williams, Katherine L. ^{[2
]}

Gonzalez, Ritela ^{[2
]}

Murray, Meredith ^{[1
]}

Sluzas, Emily ^{[1
]}

Pagano, Dan ^{[1
]}

Ajith, Sandya ^{[1
]}

Bower, Megan ^{[1
]}

Berdougo, Eli ^{[1
]}

Harris, Eva ^{[2
]}

Doranz, Benjamin J. ^{[1
]}

机构：

[1] Integral Mol Inc, Philadelphia, PA USA

[2] Univ Calif Berkeley, Sch Publ Hlth, Div Infect Dis & Vaccinol, Berkeley, CA 94720 USA

来源：

PLOS ONE | 2011年 / 6卷 / 11期

基金：

美国国家卫生研究院;

关键词：

WEST-NILE-VIRUS; PACKAGING CELL-LINE; DC-SIGN; MONOCLONAL-ANTIBODIES; INFECTION; DETERMINANTS; ASSAY; IDENTIFICATION; ENCEPHALITIS; PATHOGENESIS;

D O I：

10.1371/journal.pone.0027252

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The lack of reliable, high-throughput tools for characterizing anti-dengue virus (DENV) antibodies in large numbers of serum samples has been an obstacle in understanding the impact of neutralizing antibodies on disease progression and vaccine efficacy. A reporter system using pseudoinfectious DENV reporter virus particles (RVPs) was previously developed by others to facilitate the genetic manipulation and biological characterization of DENV virions. In the current study, we demonstrate the diagnostic utility of DENV RVPs for measuring neutralizing antibodies in human serum samples against all four DENV serotypes, with attention to the suitability of DENV RVPs for large-scale, long-term studies. DENV RVPs used against human sera yielded serotype-specific responses and reproducible neutralization titers that were in statistical agreement with Plaque Reduction Neutralization Test (PRNT) results. DENV RVPs were also used to measure neutralization titers against the four DENV serotypes in a panel of human sera from a clinical study of dengue patients. The high-throughput capability, stability, rapidity, and reproducibility of assays using DENV RVPs offer advantages for detecting immune responses that can be applied to large-scale clinical studies of DENV infection and vaccination.

引用

页数：9

共 34 条

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