Ca2+ stores in the chick embryo retina cells

被引:11
作者
Cristovao, AJ [1 ]
Capela, AN [1 ]
Carvalho, CM [1 ]
机构
[1] UNIV COIMBRA,CTR NEUROSCI COIMBRA,P-3000 COIMBRA,PORTUGAL
关键词
retina cells; Ca2+ stores; Ins(1,4,5)P-3; arachidonic acid; cyclic-ADP-ribose;
D O I
10.1016/S0898-6568(96)00116-7
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The Ca2+ stores of digitonin permeabilized chick embryo retina cells in culture were characterized, by using the fluorescence of Fluo-3 potassium salt to follow continuously the free [Ca2+] in the medium. After ATP dependent Ca2+ accumulation, the Ca2+ release was induced by several agents: 10 mu M cyclic-ADP-ribose (cADPR), 40 mu M Ins(1,4,5)P-3 10 mu M thapsigargin (Th), 25 mu M ionomycin (Ion), 15 mu M CCCP together with 4.5 mu g/ml oligomycin (CCCP/Olig), 50 mu M arachidonic acid (AA). Neither Ins(1,4,5)P-3 nor cADPR were able to mobilize Ca2+ from internal stores in these cells, but Th and AA were effective in releasing Ca2+. Four major Ca2+ stores in chick embryo retina cells were distinguished: i) the thapsigargin sensitive Ca2+ store, most likely the ER; ii) the Ca2+ store sensitive to oligomycin and CCCP, most likely the mitochondrial Ca2+ store, iii) an AA sensitive Ca2+ store, which is distinct from the previous two; and, iv) the Ca2+ store only sensitive to ionomycin. The capacities of these different Ca2+ stores of the chick embryo retina cells, relative to the total intracellular stores, are: 63.3%, 14.1%, 8.2%, for the ER, the mitochondrial and for the AA sensitive Ca2+ stores, respectively. Copyright (C) 1997 Elsevier Science Inc.
引用
收藏
页码:97 / 103
页数:7
相关论文
共 41 条
[1]  
Adler R., 1990, METHODS NEUROSCIENCE, V2, P134
[2]   INOSITOL TRISPHOSPHATE AND CALCIUM SIGNALING [J].
BERRIDGE, MJ .
NATURE, 1993, 361 (6410) :315-325
[3]   CELL SIGNALING - A TALE OF 2 MESSENGERS [J].
BERRIDGE, MJ .
NATURE, 1993, 365 (6445) :388-389
[4]   SYNERGISTIC CALCIUM-RELEASE IN THE SEA-URCHIN EGG BY RYANODINE AND CYCLIC ADP RIBOSE [J].
BUCK, WR ;
HOFFMANN, EE ;
RAKOW, TL ;
SHEN, SS .
DEVELOPMENTAL BIOLOGY, 1994, 163 (01) :1-10
[5]   BOVINE ADRENAL CHROMAFFIN CELLS CONTAIN AN INOSITOL 1,4,5-TRISPHOSPHATE-INSENSITIVE BUT CAFFEINE-SENSITIVE CA2+ STORE THAT CAN BE REGULATED BY INTRALUMINAL FREE CA2+ [J].
CHEEK, TR ;
BARRY, VA ;
BERRIDGE, MJ ;
MISSIAEN, L .
BIOCHEMICAL JOURNAL, 1991, 275 :697-701
[6]  
CRISTOVAO AJ, 1992, CELL SIGNAL, V4, P387
[7]   GABA-MEDIATED CONTROL OF GLUTAMATE-DECARBOXYLASE (GAD) IN CELL AGGREGATE CULTURE OF CHICK-EMBRYO RETINA [J].
DEMELLO, FG .
DEVELOPMENTAL BRAIN RESEARCH, 1984, 14 (01) :7-13
[8]   ARACHIDONIC ACID-INDUCED CA2+ RELEASE FROM ISOLATED SARCOPLASMIC-RETICULUM [J].
DETTBARN, C ;
PALADE, P .
BIOCHEMICAL PHARMACOLOGY, 1993, 45 (06) :1301-1309
[9]   CA2+-DEPENDENT RELEASE OF [H-3] GABA IN CULTURED CHICK RETINA CELLS [J].
DUARTE, CB ;
FERREIRA, IL ;
SANTOS, PF ;
OLIVEIRA, CR ;
CARVALHO, AP .
BRAIN RESEARCH, 1992, 591 (01) :27-32
[10]  
DUARTE CB, 1996, VISION RES, V36, P1090