Comparative effects of pravastatin-induced DNA synthesis on serum-stimulated synchronized and proliferative rat mesangial cells

被引：2

作者：

Higueruelo, S ^{[1
]}

Romero, R ^{[1
]}

机构：

[1] HOSP UNIV GERMANS TRIAS PUJOL,DEPT NEPHROL,NEPHROL RES UNIT,BADALONA 08916,SPAIN

来源：

PHARMACOLOGICAL RESEARCH | 1996年 / 34卷 / 3-4期

关键词：

pravastatin; HMG-CoA reductase inhibitors; mesangial cells; DNA synthesis; glomerulosclerosis;

D O I：

10.1006/phrs.1996.0074

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

To compare the effects of pravastatin, a 3-hydroxy-3-methylglutaryl-Coenzyme A (HMG-CoA) reductase inhibitor, on DNA synthesis in synchronized and proliferative rat glomerular mesangial cells (MCs), growth-arrested and proliferative MCs were exposed to medium containing 20% or 10% FBS, respectively, to induce mitogenesis. Different doses of pravastatin (10(-3)-10(-9) M) were added for 48 h. DNA synthesis was assessed by Bromodeoxyuridine (BrdU) incorporation. Pravastatin caused a significant (P < 0.05) dose-dependent reduction in BrdU incorporation in both serum-stimulated synchronized and proliferative MCs, but higher doses are required in the latter. (C) 1996 The Italian Pharmacological Society.

引用

页码：117 / 120

页数：4

共 27 条

[1] INDUCTION OF NODULAR SCLEROSIS BY INSULIN IN RAT MESANGIAL CELLS IN-VITRO - STUDIES OF COLLAGEN [J].

ABRASS, CK ;

SPICER, D ;

RAUGI, GJ .

KIDNEY INTERNATIONAL, 1995, 47 (01) :25-37

[2] THERAPEUTIC ADVANTAGE OF CONVERTING ENZYME-INHIBITORS IN ARRESTING PROGRESSIVE RENAL-DISEASE ASSOCIATED WITH SYSTEMIC HYPERTENSION IN THE RAT [J].

ANDERSON, S ;

RENNKE, HG ;

BRENNER, BM .

JOURNAL OF CLINICAL INVESTIGATION, 1986, 77 (06) :1993-2000

[3]

BIESENBACH G, 1992, CLIN NEPHROL, V37, P274

[4] INTERACTIONS OF LDL AND MODIFIED LDL WITH MESANGIAL CELLS AND MATRIX [J].

GUPTA, S ;

RIFICI, V ;

CROWLEY, S ;

BROWNLEE, M ;

SHAN, ZH ;

SCHLONDORFF, D .

KIDNEY INTERNATIONAL, 1992, 41 (05) :1161-1169

[5] LOVASTATIN AMELIORATES THE DEVELOPMENT OF GLOMERULOSCLEROSIS AND UREMIA IN EXPERIMENTAL NEPHROTIC SYNDROME [J].

HARRIS, KPG ;

PURKERSON, ML ;

YATES, J ;

KLAHR, S .

AMERICAN JOURNAL OF KIDNEY DISEASES, 1990, 15 (01) :16-23

[6] TREATMENT OF HYPERLIPIDEMIA WITH PROBUCOL REDUCES PROTEINURIA IN PUROMYCIN AMINONUCLEOSIDE-INDUCED NEPHROTIC RATS [J].

HIRANO, T ;

MAMO, JCL ;

OHNAMI, K ;

SUGISAKI, T .

NEPHRON, 1990, 54 (04) :358-359

[7] EFFECT OF PRAVASTATIN SODIUM, A NEW INHIBITOR OF 3-HYDROXY-3-METHYLGLUTARYL COENZYME-A REDUCTASE, ON VERY LOW-DENSITY-LIPOPROTEIN COMPOSITION AND KINETICS IN HYPERLIPIDEMIA ASSOCIATED WITH EXPERIMENTAL NEPHROSIS [J].

HIRANO, T ;

KOMURO, F ;

FURUKAWA, S ;

NAGANO, S ;

TAKAHASHI, T .

METABOLISM-CLINICAL AND EXPERIMENTAL, 1990, 39 (06) :605-609

[8]

IKEDA M, 1992, AM J PATHOL, V141, P327

[9] GLOMERULAR GROWTH IN THE REMNANT KIDNEY AFTER A CONTRALATERAL NEPHRECTOMY [J].

IKEDA, M ;

SUZUKI, M ;

MIYAZAKI, K ;

TAKEUCHI, Y ;

YAMAGUCHI, Y ;

SAKAI, S ;

KAWAGUCHI, Y ;

SAKAI, O .

NEPHRON, 1993, 65 (01) :95-99

[10] GLOMERULOSCLEROSIS INDUCED BY IN-VIVO TRANSFECTION OF TRANSFORMING GROWTH-FACTOR-BETA OR PLATELET-DERIVED GROWTH-FACTOR GENE INTO THE RAT-KIDNEY [J].

ISAKA, Y ;

FUJIWARA, Y ;

UEDA, N ;

KANEDA, Y ;

KAMADA, T ;

IMAI, E .

JOURNAL OF CLINICAL INVESTIGATION, 1993, 92 (06) :2597-2601

← 1 2 3 →