Proliferation of human osteoblastic cells and synthesis of extracellular bone matrix on biomaterials

被引：3

作者：

Schaefer, DJ ^{[1
]}

Munder, B ^{[1
]}

Voigt, M ^{[1
]}

Stark, GB ^{[1
]}

机构：

[1] Univ Freiburg, Dept Plast & Handsurg, Valley Tissue Engn Ctr, D-79106 Freiburg, Germany

来源：

BIOMATERIALS REGULATING CELL FUNCTION AND TISSUE DEVELOPMENT | 1998年 / 530卷

关键词：

D O I：

10.1557/PROC-530-105

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Primary human osteoblasts were cultered on biomaterials to develop living bone substitutes by Tissue Engineering. Monolayer cultures were established from iliac crest biopsies. Single cell suspensions were then seeded onto tricalciumphosphate (TCP) BIOBASE(R), hydroxyapatite-collagen(HAC), autoclaved cancellous bone (CB) and fibrin glue (FG) Tissucol(R). The constructs were analyzed by electronmicroscopy, Cell proliferation assay (XTT) and histology to assess adhesion, proliferation and extracellular matrix formation on the biomaterial. The osteoblastic cells survived in the fibrin glue and established a mesenchymal phenotype. They adhered to the MAC-sponges, TCP and CB. Prolifertion was seen on TCP and CB. HAC had a suppressive effect on the cells. An inmature matrix was found on TCP and a bone neomatrix on CB after 4 weeks in vitro.

引用

页码：105 / 109

页数：5