Common RNA replication signals exist among group 2 coronaviruses: evidence for in vivo recombination between animal and human coronavius molecules

被引:34
作者
Wu, HY
Guy, JS
Yoo, D
Vlasak, R
Urbach, E
Brian, DA
机构
[1] Univ Tennessee, Coll Vet Med, Dept Microbiol, Knoxville, TN 37996 USA
[2] Univ Tennessee, Coll Vet Med, Dept Pathobiol, Knoxville, TN 37996 USA
[3] N Carolina State Univ, Coll Vet Med, Dept Farm Anim Hlth & Resource Management, Raleigh, NC 27606 USA
[4] Univ Guelph, Ontario Vet Coll, Dept Pathobiol, Guelph, ON N1G 2W1, Canada
[5] Austrian Acad Sci, Inst Mol Biol, Dept Biochem, A-5020 Salzburg, Austria
关键词
group; 2; coronaviruses; RNA replication signals; RdRp promoter; DI RNA; leader switching; recombination between human and animal;
D O I
10.1016/S0042-6822(03)00511-7
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
5' and 3' UTR sequences on the coronavirus genome are known to carry cis-acting elements for DI RNA replication and presumably also virus genome replication. 5' UTR-adjacent coding sequences are also thought to harbor cis-acting elements. Here we have determined the 5' UTR and adjacent 289-nt sequences, and 3' UTR sequences, for six group 2 coronaviruses and have compared them to each other and to three previously reported group 2 members. Extensive regions of highly similar UTR sequences were found but small regions of divergence were also found indicating group 2 coronaviruses could be subdivided into those that are bovine coronavirus (BCoV)-like (BCoV, human respiratory coronavirus-OC43, human enteric coronavirus, porcine hernagglutinating encephalomyelitis virus, and equine coronavirus) and those that are murine hepatitis virus (MHV)-like (A59, 2, and JHM strains of MHV, puffinosis virus, and rat sialodacryoadenitis virus). The 3' UTRs of BCoV and MHV have been previously shown to be interchangeable. Here, a reporter-containing BCoV DI RNA was shown to be replicated by all five BCoV-Iike helper viruses and by MHV-H2 (a human cell-adapted MHV strain), a representative of the MHV-like subgroup, demonstrating group 2 common 5' and 3' replication signaling elements. BCoV DI RNA, furthermore, acquired the leader of HCoV-OC43 by leader switching, demonstrating for the first time in vivo recombination between animal and human coronavirus molecules. These results indicate that common replication signaling elements exist among group 2 coronaviruses despite a two-cluster pattern within the group and imply there could exist a high potential for recombination among group members. (C) 2003 Elsevier Inc. All rights reserved.
引用
收藏
页码:174 / 183
页数:10
相关论文
共 49 条
[1]   Episodic evolution mediates interspecies transfer of a murine coronavirus [J].
Baric, RS ;
Yount, B ;
Hensley, L ;
Peel, SA ;
Chen, W .
JOURNAL OF VIROLOGY, 1997, 71 (03) :1946-1955
[2]   Recombination and coronavirus defective interfering RNAs [J].
Brian, DA ;
Spaan, WJM .
SEMINARS IN VIROLOGY, 1997, 8 (02) :101-111
[3]  
Cavanagh D, 1997, ARCH VIROL, V142, P629
[4]   The UCUAAAC promoter motif is not required for high-frequency leader recombination in bovine coronavirus defective interfering RNA [J].
Chang, RY ;
Krishnan, R ;
Brian, DA .
JOURNAL OF VIROLOGY, 1996, 70 (05) :2720-2729
[5]   A CIS-ACTING FUNCTION FOR THE CORONAVIRUS LEADER IN DEFECTIVE INTERFERING RNA REPLICATION [J].
CHANG, RY ;
HOFMANN, MA ;
SETHNA, PB ;
BRIAN, DA .
JOURNAL OF VIROLOGY, 1994, 68 (12) :8223-8231
[6]   cis requirement for N-specific protein sequence in bovine coronavirus defective interfering RNA replication [J].
Chang, RY ;
Brian, DA .
JOURNAL OF VIROLOGY, 1996, 70 (04) :2201-2207
[7]   CYTOPLASMIC EXPRESSION SYSTEM BASED ON CONSTITUTIVE SYNTHESIS OF BACTERIOPHAGE-T7 RNA-POLYMERASE IN MAMMALIAN-CELLS [J].
ELROYSTEIN, O ;
MOSS, B .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (17) :6743-6747
[8]  
ENJUANES L, 2000, VIRUS TAXONOMY 7 REP, P834
[9]  
Felsenstein J., 1993, Phylip: Phylogeny Interference Package
[10]   Characterization of a coronavirus isolated from a diarrheic foal [J].
Guy, JS ;
Breslin, JJ ;
Breuhaus, B ;
Vivrette, S ;
Smith, LG .
JOURNAL OF CLINICAL MICROBIOLOGY, 2000, 38 (12) :4523-4526