New fluorescent probes for testing combinatorial catalysts with phosphodiesterase and esterase activities

被引：9

作者：

Caturla, F ^{[1
]}

Enjo, J ^{[1
]}

Bernabeu, MC ^{[1
]}

Le Serre, S ^{[1
]}

机构：

[1] Univ Marburg, Fachbereich Chem, D-35032 Marburg, Germany

来源：

TETRAHEDRON | 2004年 / 60卷 / 08期

关键词：

combinatorial catalysis; fluorescence; ester hydrolysis; phosphodiester hydrolysis;

D O I：

10.1016/j.tet.2003.12.032

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

Combinatorial development of new catalysts with phosphodiesterase and esterase activities requires specific fluorescent probes for rapid visual detection of hydrolytic activity. Such fluorescent probes have been synthesized with special attention to solubility in water and stability towards spontaneous hydrolysis at a given pH. The probes reported here include compound 5 based on a fluorescein fluorophore, compound 12 for FRET-detection of phosphodiester hydrolysis and compound 25 based on a quinolinium fluorophore. (C) 2003 Elsevier Ltd. All rights reserved.

引用

页码：1903 / 1911

页数：9

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