Development and characterization of microsatellite markers for Fagus crenata Blume

被引：53

作者：

Asuka, Y ^{[1
]}

Tani, N

Tsumura, Y

Tomaru, N

机构：

[1] Nagoya Univ, Lab Forest Ecol & Physiol, Grad Sch Bioagr Sci, Nagoya, Aichi 4648601, Japan

[2] Forestry & Forest Prod Res Inst, Dept Forest Genet, Tsukuba, Ibaraki 3058687, Japan

来源：

MOLECULAR ECOLOGY NOTES | 2004年 / 4卷 / 01期

关键词：

beech; gene dispersal; genetic variation; mating system; parentage analysis; simple sequence repeat;

D O I：

10.1046/j.1471-8286.2003.00583.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Microsatellite markers can yield high-resolution genetic profiles for individual identification, and for parentage analysis, when evaluating gene dispersal in populations. Fagus crenata is an important dominant species in the cool temperate forests in Japan, and although many studies on the species have been conducted the patterns of gene dispersal via pollen and seeds are poorly understood. In order to be better informed about gene dispersal in Fagus crenata, we have developed 16 new microsatellite loci from an enriched library of genomic DNA. These 16 loci were highly variable, with 3-40 alleles per locus and an expected heterozygosity value of 0.11-0.98.

引用

页码：101 / 103

页数：3

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