Identification of five gelatins by ultra performance liquid chromatography/time-of-flight mass spectrometry (UPLC/Q-TOF-MS) using principal component analysis

被引：76

作者：

Cheng, Xian-Long ^{[1
,2
]}

Wei, Feng ^{[1
]}

Xiao, Xin-Yue ^{[1
]}

Zhao, Ying-Yong ^{[3
]}

Shi, Yan ^{[1
]}

Liu, Wei ^{[1
]}

Zhang, Ping ^{[1
]}

Ma, Shuang-Cheng ^{[1
]}

Tian, Shou-Sheng ^{[4
]}

Lin, Rui-Chao ^{[1
,2
]}

机构：

[1] State Food & Drug Adm, Natl Inst Food & Drug Control, Res & Inspect Ctr Tradit Chinese Med & Ethnomed, Beijing 100050, Peoples R China

[2] Beijing Univ Chinese Med, Sch Chinese Pharm, Beijing 100102, Peoples R China

[3] Northwest Univ, Biomed Key Lab Shaanxi Prov, Xian 710069, Shaanxi, Peoples R China

[4] Shandong Dong EE Jiao Co Ltd, Liaocheng 252201, Peoples R China

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 2012年 / 62卷

基金：

中国国家自然科学基金;

关键词：

UPLC/Q-TOF-MS; Gelatin; Polypeptides; PCA; Identification; POLYMERASE-CHAIN-REACTION; MESSENGER-RNA; COLLAGEN; BOVINE; DIFFERENTIATION;

D O I：

10.1016/j.jpba.2011.12.024

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An ultra-performance liquid chromatography-quadrupole-time of flight mass spectrometry (UPLC/Q-TOF-MS) method coupled with a principal component analysis (PCA) was developed and applied toward identifying donkey-hide gelatin, bovine-hide gelatin, pig-hide gelatin, tortoise shell glue, and deerhorn glue. The UPLC-MS data of the trypsin digested samples were subjected to principal component analysis (PCA) in order to classify these five gelatins. Additionally, marker peptides given by the loadings plot of PCA were identified based on a comparison of recorded LC-MS data with a previously reported database of the corresponding gelatin variants. The results from this study indicate that the proposed method is reliable, and it has been successfully applied to the identification of variants of gelatins commonly used in Traditional Chinese Medicine (TCM). (C) 2011 Elsevier B.V. All rights reserved.

引用

页码：191 / 195

页数：5

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