Application of probe electrospray to direct ambient analysis of biological samples

被引：59

作者：

Chen, Lee Chuin ^{[1
]}

Nishidate, Kentaro ^{[1
]}

Saito, Yuta ^{[1
]}

Mori, Kunihiko ^{[1
]}

Asakawa, Daiki ^{[1
]}

Takeda, Sen ^{[2
]}

Kubota, Takeo ^{[3
,5
]}

Terada, Nobuo ^{[4
,5
]}

Hashimoto, Yutaka ^{[1
]}

Hori, Hirokazu ^{[5
]}

Hiraoka, Kenzo ^{[1
]}

机构：

[1] Univ Yamanashi, Clean Energy Res Ctr, Kofu, Yamanashi 4008511, Japan

[2] Univ Yamanashi, Fac Med, Dept Anat & Cell Biol, Chou 4093898, Japan

[3] Univ Yamanashi, Fac Med, Dept Epigenet Med, Chou 4093898, Japan

[4] Univ Yamanashi, Fac Med, Dept Mol Histol, Chou 4093898, Japan

[5] Univ Yamanashi, Interdisciplinary Grad Sch Med & Engn, Kofu, Yamanashi 4008511, Japan

来源：

RAPID COMMUNICATIONS IN MASS SPECTROMETRY | 2008年 / 22卷 / 15期

关键词：

D O I：

10.1002/rcm.3626

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Recently, we have developed probe electrospray ionization (PESI) that uses a solid needle. In this system, the probe needle moves up and down along the vertical axis by a motor-driven system. At the highest position of the probe needle, electrospray is generated by applying a high voltage. In this study, we applied PESI directly to biological samples such as urine, mouse brain, mouse liver, salmon egg, and fruits (orange, banana, etc.). Strong ion signals for almost all the samples were obtained. The amount of liquid sample picked up by the needle is as small as pL or less, making PESI a promising non-invasive technique for detecting biomolecules in living systems such as cells. Therefore, PESI may be useful as a versatile and ready-to-use semi-online analytical tool in the fields of medicine, pharmaceuticals, agriculture, food science, etc. Copyright (c) 2008 John Wiley & Sons, Ltd.

引用

页码：2366 / 2374

页数：9

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