Chemically induced aneuploidy: investigations into chromosome specific effects in mitosis

被引:11
作者
Bourner, RDP [1 ]
Parry, EM [1 ]
Parry, JM [1 ]
机构
[1] Univ Wales, Sch Biol Sci, Ctr Mol Genet & Toxicol, Swansea SA2 8PP, W Glam, Wales
关键词
aneuploidy; mitosis; aneugen; hybrid cell; chromosome specificity;
D O I
10.1016/S0027-5107(98)00113-4
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Genotoxicity studies of aneuploidy may potentially produce different results depending upon the chromosome selected for analysis if chromosome-specific sensitivities to chemical exposure exist. Any chromosome specificity characteristics that predispose to aneuploidy might interact with environmental exposures in additional different ways related to the mechanism of aneuploidy induction. Thus, we have undertaken an investigation of chromosome-specific effects using morphologically distinct chromosomes in a hybrid cell line. We were able to identify eight different chromosomes simultaneously by dual colour FISH analysis in controls and in cells exposed to a range of griseofulvin concentrations. Certain chromosomes were more frequently involved in aneuploidy, but no simple relationship between chromosome organisation and sensitivity emerged apart from the over-representation of the alien human chromosome. Aneuploidy was detected at higher frequencies in interphase cells compared with metaphase cells. Overall the data indicate that chemically induced aneuploidy may be detected for a variety of chromosomes and cell types using both interphase and metaphase protocols. However, the data obtained should be used with care in the hazard evaluation of chemical aneugens. (C) 1998 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:191 / 197
页数:7
相关论文
共 14 条
[1]   USE OF A HUMAN X MOUSE HYBRID CELL-LINE TO DETECT ANEUPLOIDY INDUCED BY ENVIRONMENTAL CHEMICALS [J].
ATHWAL, RS ;
SANDHU, SS .
MUTATION RESEARCH, 1985, 149 (01) :73-81
[2]  
BOURNER RDP, 1998, THESIS U WALES SWANS
[3]  
Caria H, 1996, TERATOGEN CARCIN MUT, V16, P243, DOI 10.1002/(SICI)1520-6866(1996)16:5<243::AID-TCM1>3.0.CO
[4]  
2-I
[5]   CHEMICALLY-INDUCED ANEUPLOIDY IN MAMMALIAN-CELLS IN CULTURE [J].
GALLOWAY, SM ;
IVETT, JL .
MUTATION RESEARCH, 1986, 167 (1-2) :89-105
[6]   X chromosome inactivation and micronuclei in normal and Turner individuals [J].
Hando, JC ;
Tucker, JD ;
Davenport, M ;
Tepperberg, J ;
Nath, J .
HUMAN GENETICS, 1997, 100 (5-6) :624-628
[7]  
Hassold T, 1996, ENVIRON MOL MUTAGEN, V28, P167
[8]   OLIGONUCLEOTIDE-PRIMING METHODS FOR THE CHROMOSOME-SPECIFIC LABELING OF ALPHA-SATELLITE DNA INSITU [J].
KOCH, JE ;
KOLVRAA, S ;
PETERSEN, KB ;
GREGERSEN, N ;
BOLUND, L .
CHROMOSOMA, 1989, 98 (04) :259-265
[9]   THE USE OF FLUORESCENCE IN-SITU HYBRIDIZATION WITH A BETA-SATELLITE DNA-PROBE FOR THE DETECTION OF ACROCENTRIC CHROMOSOMES IN VANADIUM-INDUCED MICRONUCLEI [J].
MIGLIORE, L ;
SCARPATO, R ;
FALCO, P .
CYTOGENETICS AND CELL GENETICS, 1995, 69 (3-4) :215-219
[10]   COMPARISONS OF TESTS FOR ANEUPLOIDY [J].
PARRY, JM ;
PARRY, EM .
MUTATION RESEARCH, 1987, 181 (02) :267-287