Analysis of epidermal-type transglutaminase (TGase 3) expression in mouse tissues and cell lines

被引：57

作者：

Hitomi, K ^{[1
]}

Horio, Y

Ikura, K

Yamanishi, K

Maki, M

机构：

[1] Nagoya Univ, Grad Sch Bioagr Sci, Dept Appl Mol Biosci, Nagoya, Aichi 4648601, Japan

[2] Kyoto Inst Technol, Dept Appl Biol, Kyoto 6068585, Japan

[3] Kyoto Prefectural Univ Med, Dept Dermatol, Kamigyou Ku, Kyoto 6028566, Japan

来源：

INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY | 2001年 / 33卷 / 05期

关键词：

transglutaminase; epidermis; RT-PCR; keratinocyte; MKN-1;

D O I：

10.1016/S1357-2725(01)00033-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

In the formation of the cornified cell envelope in the epidermis, epidermal-type transglutaminase (TGase 3) cross-links a variety of structural proteins. However, its expression in other tissue has not been investigated. Furthermore, no cell line expressing TGase 3 has been found. The tissue distribution of TGase 3 in mice was investigated using reverse-transcription polymerase chain reaction (RT-PCR) and Western blotting analyses. TGase 3 mRNA was expressed in the brain, stomach, spleen, small intestine, testis, skeletal muscle and skin. The stomach and testis expressed TGase 3 protein in size similar to that observed in the epidermis. Screening various cell lines, a gastric human cancer cell line, MKN-1 and mouse neuroblast cell line, neuro2a, were found to express TGase 3. (C) 2001 Elsevier Science Ltd. All rights reserved.

引用

页码：491 / 498

页数：8

共 35 条

[1]

Isolation of a cDNA encoding a novel member of the transglutaminase gene family from human keratinocytes - Detection and identification of transglutaminase gene products based on reverse transcription polymerase chain reaction with degenerate primers [J].