Characterization of a hexokinase encoding cDNA of the parasitic nematode Haemonchus contortus

被引：8

作者：

Schmitt-Wrede, HP

Waldraff, A

Krücken, J

Harder, A

Wunderlich, F

机构：

[1] Univ Dusseldorf, Div Mol Parasitol, D-40225 Dusseldorf, Germany

[2] Univ Dusseldorf, Ctr Biol & Med Res, D-40225 Dusseldorf, Germany

[3] Bayer AG, Inst Parasitol, Div Anim Hlth, D-51368 Leverkusen, Germany

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 1999年 / 1444卷 / 03期

关键词：

hexokinase; cDNA; (gastrointestinal nematode; Haemonchus contortus);

D O I：

10.1016/S0167-4781(99)00009-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The nematode Haemonchus contortus is an important parasite of cattle and sheep. We describe here the cloning of a cDNA encoding a 53 kDa hexokinase (EC 2.7.1.1). The deduced protein shows 73% identity to a 50 kDa hexokinase deduced from a Caenorhabditis elegans cosmid. Alignment with mammalian hexokinases reveals two short amino acid insertions in the H. contortus hexokinase. Software tools for structural protein analysis (ExPASy server, Geneva) localize these insertions on the surface of the molecule, suggesting these surface changes as potential target sites for chemotherapeutic drugs. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：439 / 444

页数：6

共 16 条

[1] STRUCTURE OF A COMPLEX BETWEEN YEAST HEXOKINASE-A AND GLUCOSE .2. DETAILED COMPARISONS OF CONFORMATION AND ACTIVE-SITE CONFIGURATION WITH THE NATIVE HEXOKINASE-B MONOMER AND DIMER [J].