Immunochemical Analysis of Glial Fibrillary Acidic Protein as a Tool to Assess Astroglial Reaction in Experimental C6 Glioma

被引：14

作者：

Yusubalieva, G. M. ^{[1
]}

Baklaushev, V. P. ^{[1
]}

Gurina, O. I. ^{[1
]}

Tsitrin, E. B. ^{[2
]}

Chekhonin, V. P. ^{[1
]}

机构：

[1] Russian Acad Sci, VP Serbskii Natl Res Ctr Social & Forens Psychiat, Dept Fundamental & Appl Neurobiol, Fed Agcy Hlth Care & Social Dev, Moscow, Russia

[2] Russian Acad Sci, NK Koltsov Dev Biol Inst, Moscow, Russia

来源：

BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE | 2010年 / 149卷 / 01期

关键词：

glial fibrillary acidic protein (GFAP); glioma C6; enzyme immunoassay; reactive astrogliosis; CELLS; INVASION; NEURONS; DISEASE; TUMORS;

D O I：

10.1007/s10517-010-0890-7

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

100103 [病原生物学]; 100218 [急诊医学];

摘要：

In experiments on Wistar rats with experimental C6 glioma, the immunohistochemical features of the astroglial reaction over 30 days after implantation were characterized. The formation of a glial border consisting of GFAP-positive reactive astrocytes at the periphery of C6 glioma was observed on postimplantation day 3 and until the death of the experimental animals. Reactive astrocytes encompassed not only the primary gliomal focus, but all tumor invasion foci. Quantitative assessment of astroglial reaction around glioma was carried out with immunofluorescent assay of glial. brillary acidic protein (GFAP) on cerebral sections. The size of glioma and necrotic foci were analyzed morphometrically in parallel with enzyme immunoassay of serum GFAP. A correlation between morphometric indices of glioma and serum level of GFAP was found. It was concluded that serum concentration of GFAP correlated with the size of intracranial glioma, necrotic foci, and most strongly with the degree of reactive astrogliosis. Monitoring of the level of serum GFAP can serve as an additional diagnostic index reporting the state of intracranial glioma.

引用

页码：125 / 130

页数：6

共 21 条

[1]

Glial pathology but absence of apoptotic nigral neurons in long-standing Parkinson's disease [J].

Banati, RB ;

Daniel, SE ;

Blunt, SB .

MOVEMENT DISORDERS, 1998, 13 (02) :221-227

[2]

Berezin V. A., 1990, SPECIFIC PROTEINS NE

[3]

Serum levels of glial fibrillary acidic protein correlate to tumour volume of high-grade gliomas [J].

Brommeland, T. ;

Rosengren, L. ;

Fridlund, S. ;

Hennig, R. ;

Isaksen, V. .

ACTA NEUROLOGICA SCANDINAVICA, 2007, 116 (06) :380-384

[4]

Chekhonin V.P., 2007, KLETOCHNYE TEKHNOLOG, P65

[5]

CHEKHONIN VP, 2000, IMMUNOCHEMICAL ANAL

[6]

MOLECULAR PROFILE OF REACTIVE ASTROCYTES - IMPLICATIONS FOR THEIR ROLE IN NEUROLOGIC DISEASE [J].

EDDLESTON, M ;

MUCKE, L .

NEUROSCIENCE, 1993, 54 (01) :15-36

[7]

Glial fibrillary acidic protein: GFAP-thirty-one years (1969-2000) [J].

Eng, LF ;

Ghirnikar, RS ;

Lee, YL .

NEUROCHEMICAL RESEARCH, 2000, 25 (9-10) :1439-1451

[8]

Serum glial fibrillary acidic protein as a biomarker for intracerebral haemorrhage in patients with acute stroke [J].

Foerch, C ;

Curdt, I ;

Yan, B ;

Dvorak, F ;

Hermans, M ;

Berkefeld, J ;

Raabe, A ;

Neumann-Haefelin, T ;

Steinmetz, H ;

Sitzer, M .

JOURNAL OF NEUROLOGY NEUROSURGERY AND PSYCHIATRY, 2006, 77 (02) :181-184

[9]

Goldman R. D., 1990, CELLULAR MOL BIOL IN, P3

[10]

Huang RC, 2002, CANCER RES, V62, P2806

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