Resistance to Aspergillus flavus in corn kernels is associated with a 14-kDa protein

被引：96

作者：

Chen, ZY

Brown, RL ^{[1
]}

Lax, AR

Guo, BZ

Cleveland, TE

Russin, JS

机构：

[1] Louisiana State Univ, Ctr Agr, Dept Plant Pathol & Crop Physiol, Baton Rouge, LA 70803 USA

[2] USDA ARS, So Reg Res Ctr, New Orleans, LA 70179 USA

[3] USDA ARS, Plant Resistance Germplasm Enhancement Res Unit, Tifton, GA 31793 USA

来源：

PHYTOPATHOLOGY | 1998年 / 88卷 / 04期

关键词：

antifungal protein;

D O I：

10.1094/PHYTO.1998.88.4.276

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Corn genotypes resistant or susceptible to Aspergillus flavus were extracted for protein analysis using a pH 2.8 buffer. The profile of protein extracts revealed that a 14-kDa protein is present in relatively high concentration in kernels of seven resistant corn genotypes, but is absent or present only in low concentration in kernels of six susceptible ones. The N-terminal sequence of this 14-kDa protein showed 100% homology to a corn trypsin inhibitor. The 14-kDa protein purified from resistant varieties also demonstrated in vitro inhibition of both trypsin activity and the growth of A. flavus. This is the first demonstration of antifungal activity of a corn 14-kDa trypsin inhibitor protein. The expression of this protein among tested genotypes may be related to their difference in resistance to A. flavus infection and subsequent aflatoxin contamination.

引用

页码：276 / 281

页数：6

共 40 条

[1]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[2] DETERMINATION OF RESISTANCE TO AFLATOXIN PRODUCTION IN MAIZE KERNELS AND DETECTION OF FUNGAL COLONIZATION USING AN ASPERGILLUS-FLAVUS TRANSFORMANT EXPRESSING ESCHERICHIA-COLI BETA-GLUCURONIDASE [J].