Thermal stability of diazonium derived and thiol-derived layers on gold for application in genosensors

被引：52

作者：

Civit, Laia ^{[1
]}

Fragoso, Alex ^{[1
]}

O'Sullivan, Ciara K. ^{[1
,2
]}

机构：

[1] Univ Rovira & Virgili, Dept Engn Quim, Tarragona, Spain

[2] ICREA, Barcelona 08010, Spain

来源：

ELECTROCHEMISTRY COMMUNICATIONS | 2010年 / 12卷 / 08期

关键词：

Thermal stability; Diazonium salts; Self-assembled monolayers; Gold electrode; SELF-ASSEMBLED MONOLAYERS; STRUCTURAL-CHARACTERIZATION; DNA; IMMOBILIZATION; SURFACE;

D O I：

10.1016/j.elecom.2010.05.020

中图分类号：

O646 [电化学、电解、磁化学];

学科分类号：

081704 ;

摘要：

DNA analysis using variable temperature technologies such as melting curve analysis, high temperature mutant discrimination or immobilised PCR relies upon the use of immobilised probes that need to be stable over a wide temperature range. In this work a comparison of the thermal stability of gold surfaces modified with alkanethiol and diazonium salt derived layers is presented. Electrochemical impedance spectroscopy and cyclic voltammetry were used to characterize the electrode arrays. The applicability of the surfaces for DNA sensing was demonstrated following the thermal treatment, with the diazonium-modified surface being markedly more stable. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：1045 / 1048

页数：4

共 21 条

[1] FORMATION OF MONOLAYER FILMS BY THE SPONTANEOUS ASSEMBLY OF ORGANIC THIOLS FROM SOLUTION ONTO GOLD [J].

BAIN, CD ;

TROUGHTON, EB ;

TAO, YT ;

EVALL, J ;

WHITESIDES, GM ;

NUZZO, RG .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1989, 111 (01) :321-335

[2] Thermal treatment of n-alkanethiolate monolayers on gold, as observed by infrared spectroscopy [J].

Bensebaa, F ;

Ellis, TH ;

Badia, A ;

Lennox, RB .

LANGMUIR, 1998, 14 (09) :2361-2367

[3] Self-assembled monolayers stabilized by three-dimensional networks of hydrogen bonds [J].

Clegg, RS ;

Reed, SM ;

Hutchison, JE .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1998, 120 (10) :2486-2487

[4] A versatile method for direct and covalent immobilization of DNA and proteins on biochips [J].

Corgier, Benjamin P. ;

Laurent, Alain ;

Perriat, Pascal ;

Blum, Loic J. ;

Marquette, Christophe A. .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2007, 46 (22) :4108-4110

[5] THERMAL-STABILITY OF SELF-ASSEMBLED MONOLAYERS [J].

DELAMARCHE, E ;

MICHEL, B ;

KANG, H ;

GERBER, C .

LANGMUIR, 1994, 10 (11) :4103-4108

[6] Robust and efficient synthetic method for forming DNA microarrays [J].

Dolan, PL ;

Wu, Y ;

Ista, LK ;

Metzenberg, RL ;

Nelson, MA ;

Lopez, GP .

NUCLEIC ACIDS RESEARCH, 2001, 29 (21) :107-107

[7] BLOCKING ORIENTED MONOLAYERS OF ALKYL MERCAPTANS ON GOLD ELECTRODES [J].

FINKLEA, HO ;

AVERY, S ;

LYNCH, M ;

FURTSCH, T .

LANGMUIR, 1987, 3 (03) :409-413

[8] Electron permeable self-assembled monolayers of dithiolated aromatic scaffolds on gold for biosensor applications [J].

Fragoso, Alex ;

Laboria, Noemi ;

Latta, Daniel ;

O'Sullivan, Ciara K. .

ANALYTICAL CHEMISTRY, 2008, 80 (07) :2556-2563

[9] Self-assembled monolayers composed of aromatic thiols on gold: Structural characterization and thermal stability in solution [J].

Garg, N ;

Carrasquillo-Molina, E ;

Lee, TR .

LANGMUIR, 2002, 18 (07) :2717-2726

[10] Selective immobilization of DNA and antibody probes on electrode arrays: Simultaneous electrochemical detection of DNA and protein on a single platform [J].

Harper, Jason C. ;

Polsky, Ronen ;

Wheeler, David R. ;

Dirk, Shawn M. ;

Brozik, Susan M. .

LANGMUIR, 2007, 23 (16) :8285-8287

← 1 2 3 →