Polysaccharide scaffolds prepared by crosslinking of polysaccharides with chitosan or proteins for cell growth

被引：17

作者：

Ehrenfreund-Kleinman, T

Domb, AJ ^{[1
]}

Golenser, J

机构：

[1] Hebrew Univ Jerusalem, Fac Med, Sch Pharm, Dept Med Chem & Nat Prod, IL-91120 Jerusalem, Israel

[2] Hebrew Univ Jerusalem, Hadassah Med Sch, Dept Parasitol, IL-91120 Jerusalem, Israel

来源：

JOURNAL OF BIOACTIVE AND COMPATIBLE POLYMERS | 2003年 / 18卷 / 05期

关键词：

tissue engineering; polysaccharides; arabinogalactan; cross-linked sponges;

D O I：

10.1177/0883911503038234

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The synthesis and characterization of sponges composed of polysaccharides crosslinked with different amine-containing natural polymers for the use as cell carriers is described. Sponges based on arabinogalactan, dextran, and amylose were synthesized by crosslinking with chitosan, or with the protein: gelatin, or bovine serum albumin. Highly porous sponges that rapidly absorbed water with little change in size were obtained. The degradation rate of the sponges was varied by controlling the oxidation with periodate or perchlorite for different times and ratios, in order to tailor the sponges for use as cell carriers in tissue engineering. The sponges performed well as a platform for the growth of bEnd2 cells. The chitosan based sponges were the most effective and cell compatible.

引用

页码：323 / 338

页数：16

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