Cl channel blockers inhibit the volume-activated efflux of Cl and taurine in cultured neurons

被引:42
作者
SanchezOlea, R [1 ]
Morales, M [1 ]
Garcia, O [1 ]
PasantesMorales, H [1 ]
机构
[1] NATL AUTONOMOUS UNIV MEXICO, INST FISIOL CELULAR, MEXICO CITY 04510, DF, MEXICO
来源
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY | 1996年 / 270卷 / 06期
关键词
chloride channels; volume regulation; polyunsaturated fatty acids; cerebellar neurons;
D O I
10.1152/ajpcell.1996.270.6.C1703
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The effects of the Cl channel blockers 5-nitro-2-(3-phenylpropylamino)benzoic acid (NPPB), 1,9-dideoxyforskolin (DDF), dipyridamole, and niflumic acid and of the polyunsaturated fatty acids arachidonic, linolenic, and linoleic acids on regulatory volume decrease (RVD) and associated I-125 and [H-3]taurine fluxes in cultured rat cerebellar granule neurons were examined. Dose-response curves of NPPB, DDF, and dipyridamole showed 20-100% inhibition of RVD and osmolyte fluxes. Niflumic acid was less potent, requiring 150-600 mu M to show effects of this magnitude. The polyunsaturated fatty acids (5-20 mu M) inhibited 80-90% RVD and osmolyte fluxes, with arachidonic acid exhibiting the most potent effect. The volume-associated taurine efflux was somewhat higher in younger neurons, but the pharmacological sensitivity was essentially the same in immature and mature cells. The effects of all tested drugs on I-125 and [H-3]taurine fluxes were remarkably similar, indicating a close pharmacological sensitivity of the transport mechanism for the two osmolytes. This is in line with the suggestion of a common pathway for the volume-associated release of Cl and amino acids functioning as osmolytes.
引用
收藏
页码:C1703 / C1708
页数:6
相关论文
共 25 条
[1]   ANION CHANNELS FOR AMINO-ACIDS IN MDCK CELLS [J].
BANDERALI, U ;
ROY, G .
AMERICAN JOURNAL OF PHYSIOLOGY, 1992, 263 (06) :C1200-C1207
[2]   VOLUME-ACTIVATED TAURINE EFFLUX FROM SKATE ERYTHROCYTES - POSSIBLE BAND-3 INVOLVEMENT [J].
GOLDSTEIN, L ;
BRILL, SR .
AMERICAN JOURNAL OF PHYSIOLOGY, 1991, 260 (05) :R1014-R1020
[3]   REGIONS INVOLVED IN THE OPENING OF CIC-2 CHLORIDE CHANNEL BY VOLTAGE AND CELL-VOLUME [J].
GRUNDER, S ;
THIEMANN, A ;
PUSCH, M ;
JENTSCH, TJ .
NATURE, 1992, 360 (6406) :759-762
[4]   MEMBRANE MECHANISMS IN VOLUME AND PH REGULATION IN VERTEBRATE CELLS [J].
HOFFMANN, EK ;
SIMONSEN, LO .
PHYSIOLOGICAL REVIEWS, 1989, 69 (02) :315-382
[5]   DIRECT MODULATION OF SECRETORY CHLORIDE CHANNELS BY ARACHIDONIC AND OTHER CIS UNSATURATED FATTY-ACIDS [J].
HWANG, TC ;
GUGGINO, SE ;
GUGGINO, WB .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (15) :5706-5709
[6]   VOLUME-SENSITIVE ANION CHANNELS MEDIATE SWELLING-ACTIVATED INOSITOL AND TAURINE EFFLUX [J].
JACKSON, PS ;
STRANGE, K .
AMERICAN JOURNAL OF PHYSIOLOGY, 1993, 265 (06) :C1489-C1500
[7]   MOLECULAR PHYSIOLOGY OF ANION CHANNELS [J].
JENTSCH, TJ .
CURRENT OPINION IN CELL BIOLOGY, 1994, 6 (04) :600-606
[8]  
KIMELBERG HK, 1990, J NEUROSCI, V10, P1583
[9]  
KIRK J, 1994, J BIOL CHEM, V269, P29389
[10]  
KIRK K, 1992, J BIOL CHEM, V267, P23475