Incidence of staphylococcal colonization and of the 753Q Toll-like receptor 2 variant in nasal polyposis

被引:25
作者
Sachse, Florian [1 ]
Becker, Karsten [2 ]
Rudack, Claudia [1 ]
机构
[1] Univ Munster, Dept Otorhinolaryngol Head & Neck Surg, D-48149 Munster, Germany
[2] Univ Munster, Inst Med Microbiol, D-48149 Munster, Germany
关键词
Bacteria; bacteriology; chronic sinusitis; innate immunity; nasal polyposis; R753Q; single nucleotide polymorphism; SNP; Staphylococcus aureus; TLR; CHRONIC RHINOSINUSITIS; CHRONIC SINUSITIS; INNATE IMMUNITY; POLYMORPHISM; DISEASE; GENE; INFLAMMATION; EXPRESSION; INFECTION; RESPONSES;
D O I
10.2500/ajra.2010.24.3416
中图分类号
R76 [耳鼻咽喉科学];
学科分类号
100213 ;
摘要
Background: The impact of Staphylococcus aureus on the development of chronic sinusitis with nasal polyps (nasal polyposis [NP]) is a controversial discussion because different S. aureus colonization rates have been reported. Aside from the presence of a microbial stimulus, elements of innate immunity such as Toll-like receptors (TLRs) and/or impaired TLR function could be relevant for the development of this disease. Because the 753Q TLR2 variant may predispose to staphylococcal infection, we simultaneously analyzed staphylococcal colonization and the R753Q TLR2 single nucleotide polymorphism (SNP) in NP. Methods: Sixty-eight patients with NP (47 men and 21 women; mean age [+/- SD], 51.8 years [16.3]) and 51 controls (32 men and 19 women; mean age [+/- SD], 36.3 years [12.2]) were included. Patient characteristics studied included status of allergy, asthma, aspirin intolerance, and endoscopic and CT polyp score. For detection of bacteria, standard procedures of bacteriology and 16S rRNA gene sequencing were used. The R753Q TLR2 polymorphism was studied by allelic discrimination assay. Results: Overall, 128 isolates were cultured from 68 NP specimens, with Staphylococcus epidermidis and S. aureus being the most frequent bacterial isolates. Other bacterial species were infrequently detected. Fifty-nine isolates were cultured from 51 controls. Similarly, S. epidermidis and S. aureus were the most frequent bacterial isolates. S. aureus colonization was significantly increased in NP (p < 0.05). However, SNP genotyping results showed no association of the 753Q TLR2 variant with NP. Conclusion: Although S. aureus detection was increased in NP, nasal polyp pathology is not related to the 753Q TLR2 variant. (Am J Rhinol Allergy 24, e10-e13, 2010; doi: 10.2500/ajra.2010.24.3416)
引用
收藏
页码:E10 / E13
页数:4
相关论文
共 26 条
[1]  
Araujo Elisabeth, 2007, Braz J Otorhinolaryngol, V73, P549
[2]  
Bachert C, 2007, Chem Immunol Allergy, V93, P214, DOI 10.1159/000100897
[3]   Role of staphylococcal superantigens in upper airway disease [J].
Bachert, Claus ;
Zhang, Nan ;
Patou, Joke ;
van Zele, Thibaut ;
Gevaert, Philippe .
CURRENT OPINION IN ALLERGY AND CLINICAL IMMUNOLOGY, 2008, 8 (01) :34-38
[4]  
Baron EJ, 2007, MANUAL OF CLINICAL MICROBIOLOGY, 9TH ED, P1
[5]   Development and evaluation of a quality-controlled ribosomal sequence database for 16S ribosomal DNA-based identification of Staphylococcus species [J].
Becker, K ;
Harmsen, D ;
Mellmann, A ;
Meier, C ;
Schumann, P ;
Peters, G ;
von Eiff, C .
JOURNAL OF CLINICAL MICROBIOLOGY, 2004, 42 (11) :4988-4995
[6]   Impact of a molecular approach to improve the microbiological diagnosis of infective heart valve endocarditis [J].
Breitkopf, C ;
Hammel, D ;
Scheld, HH ;
Peters, G ;
Becker, K .
CIRCULATION, 2005, 111 (11) :1415-1421
[7]   Toll-like receptors in the pathogenesis of human disease [J].
Cook, DN ;
Pisetsky, DS ;
Schwartz, DA .
NATURE IMMUNOLOGY, 2004, 5 (10) :975-979
[8]   Staphylococcal infection and toxin production in chronic rhinosinusitis [J].
El Fiky, Lobna M. ;
Khamis, Nagwa ;
Mostafa, Badr El Din ;
Adly, Ahmed M. .
AMERICAN JOURNAL OF RHINOLOGY & ALLERGY, 2009, 23 (03) :264-267
[9]   Altered expression of genes associated with innate immunity and inflammation in recalcitrant rhinosinusitis with polyps [J].
Lane, AP ;
Truong-Tran, QA ;
Schleimer, RP .
AMERICAN JOURNAL OF RHINOLOGY, 2006, 20 (02) :138-144
[10]   A novel polymorphism in the toll-like receptor 2 gene and its potential association with staphylococcal infection [J].
Lorenz, E ;
Mira, JP ;
Cornish, KL ;
Arbour, NC ;
Schwartz, DA .
INFECTION AND IMMUNITY, 2000, 68 (11) :6398-6401