Structure-activity relationship studies of protective function of nitroxides in Fenton system

被引：19

作者：

Glebska, J

Pulaski, L

Gwozdzinski, K

Skolimowski, J

机构：

[1] Univ Lodz, Dept Mol Biophys, PL-90237 Lodz, Poland

[2] Univ Lodz, Dept Organ Chem, PL-90136 Lodz, Poland

来源：

BIOMETALS | 2001年 / 14卷 / 02期

关键词：

deoxyribose; Fenton reaction; nitroxides; structure-activity relationship; TBARS;

D O I：

10.1023/A:1016689607579

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The aim of this study was to evaluate the effect of piperidine nitroxides and their amine precursors on deoxyribose oxidation in the Fenton system. Protecting activity of nitroxides was found to be concentration-dependent and strongly influenced by ring substituents, while secondary amines did not provide any protection. The reported results suggest a mechanism of nitroxide action through iron oxidation rather than through direct scavenging of hydroxyl radicals. Moreover, presented data point to the danger of interference of nitroxides during the TBARS assay procedure.

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页码：159 / 170

页数：12

相关论文

共 39 条

[1] EFFECTS OF INDOLINIC AND QUINOLINIC AMINOXYLS ON PROTEIN AND LIPID-PEROXIDATION OF RAT-LIVER MICROSOMES [J].

ANTOSIEWICZ, J ;

POPINIGIS, J ;

WOZNIAK, M ;

DAMIANI, E ;

CARLONI, P ;

GRECI, L .

FREE RADICAL BIOLOGY AND MEDICINE, 1995, 18 (05) :913-917

[2] BINDING OF IRON(II) IONS TO THE PENTOSE SUGAR 2-DEOXYRIBOSE [J].

ARUOMA, OI ;

CHAUDHARY, SS ;

GROOTVELD, M ;

HALLIWELL, B .

JOURNAL OF INORGANIC BIOCHEMISTRY, 1989, 35 (02) :149-155

[3]

ARUOMA OI, 1994, METHOD ENZYMOL, V233, P57

[4] Kinetics of nitroxide reaction with iron(II) [J].

Bar-On, P ;

Mohsen, M ;

Zhang, RL ;

Feigin, E ;

Chevion, M ;

Samuni, A .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1999, 121 (35) :8070-8073

[5] INHIBITION OF HYDROXYL RADICAL PRODUCTION BY LACTOBIONATE, ADENINE, AND TEMPOL [J].

CHARLOUX, C ;

PAUL, M ;

LOISANCE, D ;

ASTIER, A .

FREE RADICAL BIOLOGY AND MEDICINE, 1995, 19 (05) :699-704

[6] The effects of nitroxide radicals on oxidative DNA damage [J].

Damiani, E ;

Kalinska, B ;

Canapa, A ;

Canestrari, S ;

Wozniak, M ;

Olmo, E ;

Greci, L .

FREE RADICAL BIOLOGY AND MEDICINE, 2000, 28 (08) :1257-1265

[7] THE EFFECT OF MYOGLOBIN ON THE STABILITY OF THE HYDROXYL-RADICAL ADDUCTS OF 5,5-DIMETHYL-1-PYROLLINE-N-OXIDE (DMPO), 3,3,5,5-TETRAMETHYL-1-PYROLLINE-N-OXIDE (TMPO) AND 1-ALPHA-PHENYL-TERT-BUTYL NITRONE (PBN) IN THE PRESENCE OF HYDROGEN-PEROXIDE [J].

DEBONO, D ;

YANG, WD ;

SYMONS, MCR .

FREE RADICAL RESEARCH, 1994, 20 (05) :327-332

[8] RADIATION EFFECTS ON AQUEOUS-SOLUTIONS OF NITROXYL FREE-RADICAL TMPN(2,2,6,6-TETRAMETHYL-4-PIPERIDINOL-N-OXYL) [J].

DEFFNER, U ;

SCHIMMACK, W .

INTERNATIONAL JOURNAL OF RADIATION BIOLOGY, 1976, 29 (01) :71-75

[9] FORMATION OF LONG-LIVED HYDROXYL FREE-RADICAL ADDUCTS OF PROLINE AND HYDROXYPROLINE IN A FENTON REACTION [J].

FLOYD, RA ;

ZSNAGY, I .

BIOCHIMICA ET BIOPHYSICA ACTA, 1984, 790 (01) :94-97

[10] COMPARATIVE SUGAR DEGRADATION BY (OH)(CENTER-DOT) PRODUCED BY THE IRON-DRIVEN FENTON REACTION AND GAMMA-RADIOLYSIS [J].

FRANZINI, E ;

SELLAK, H ;

HAKIM, J ;

PASQUIER, C .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1994, 309 (02) :261-265

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