Determination of trans-resveratrol in plasma by HPLC

被引:80
作者
Juan, ME
Lamuela-Raventós, RM
de la Torre-Boronat, MC
Planas, JM
机构
[1] Univ Barcelona, Fac Farm, Dept Nutr & Bromatol, E-08028 Barcelona, Spain
[2] Univ Barcelona, Fac Farm, Dept Fisiol, Div 4, E-08028 Barcelona, Spain
关键词
D O I
10.1021/ac9808831
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
trans-Resveratrol (trans-3,5,4'-trihydroxystilbene), a phenolic compound present in grapes, wines, and peanuts, has been reported to have health benefits including anticarcinogenic effects and protection against cardiovascular diseases. Despite its importance, little is known about its bioavailability in both humans and animals, A fundamental step for this evaluation consisted in measuring-this stilbene in blood, In the present work, a simple and rapid HPLC method with diode array-UV detection has been developed. Resveratrol contained in plasma was purified by solid-phase extraction using a Cls cartridge. The sample was rinsed with water and methanol-water (25:75 v/v), and trans-resveratrol was finally eluted with methanol. The collected fraction was evaporated under nitrogen and analyzed by HPLC. The method was validated by obtaining a linear correlation, a detection limit of 20 mu g/L, and a good precision with a coefficient of variation of 2.85%, trans-Resveratrol administered orally to rats was detected in plasma. With this procedure, excellent separation of trans-resveratrol is achieved, thus allowing a rapid analysis of the sample for absorption, distribution, and metabolism studies.
引用
收藏
页码:747 / 750
页数:4
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