Circular RNAs Are the Predominant Transcript Isoform from Hundreds of Human Genes in Diverse Cell Types

被引：1981

作者：

Salzman, Julia ^{[1
]}

Gawad, Charles ^{[1
,3
]}

Wang, Peter Lincoln ^{[1
]}

Lacayo, Norman ^{[3
]}

Brown, Patrick O. ^{[1
,2
]}

机构：

[1] Stanford Univ, Dept Biochem, Sch Med, Stanford, CA 94305 USA

[2] Stanford Univ, Howard Hughes Med Inst, Sch Med, Stanford, CA 94305 USA

[3] Stanford Univ, Dept Pediat Hematol Oncol, Sch Med, Stanford, CA 94305 USA

来源：

PLOS ONE | 2012年 / 7卷 / 02期

基金：

美国国家科学基金会;

关键词：

MESSENGER-RNA; MOUSE TESTIS; EXON; SRY; EXPRESSION;

D O I：

10.1371/journal.pone.0030733

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

070301 [无机化学]; 070403 [天体物理学]; 070507 [自然资源与国土空间规划学]; 090105 [作物生产系统与生态工程];

摘要：

Most human pre-mRNAs are spliced into linear molecules that retain the exon order defined by the genomic sequence. By deep sequencing of RNA from a variety of normal and malignant human cells, we found RNA transcripts from many human genes in which the exons were arranged in a non-canonical order. Statistical estimates and biochemical assays provided strong evidence that a substantial fraction of the spliced transcripts from hundreds of genes are circular RNAs. Our results suggest that a non-canonical mode of RNA splicing, resulting in a circular RNA isoform, is a general feature of the gene expression program in human cells.

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页数：12

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