Proteomic approaches to the analysis of early events in colony-stimulating factor-1 signal transduction

被引:60
作者
Yeung, YG [1 ]
Stanley, ER [1 ]
机构
[1] Albert Einstein Coll Med, Dept Dev & Mol Biol, Bronx, NY 10461 USA
关键词
D O I
10.1074/mcp.R300009-MCP200
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The exposure of cells to growth factors leads to the rapid tyrosine phosphorylation of proteins that play critical roles in initiating cellular responses. These proteins are associated with other nontyrosine-phosphorylated proteins. Together, they represent less than 0.02% of the total cellular protein. To study their functions in growth factor signaling it is necessary to establish their identity, post-translational modifications, and interactions. We have focused on the characterization of this group of proteins during the early response of macrophages to the macrophage growth factor, colony-stimulating factor-1 (CSF-1). We review here the development of approaches to analysis of the rapid CSF-1-induced changes in the CSF-1 receptor tyrosine kinase and phosphotyrosyl signaling complexes. Recent advances in mass spectrometry technology are greatly facilitating the characterization of such complexes. These methods strongly support and enhance genetic approaches that are being used to analyze the function of individual signaling components and pathways.
引用
收藏
页码:1143 / 1155
页数:13
相关论文
共 67 条
[1]   SEQUENCE REQUIREMENTS FOR BINDING OF SRC FAMILY TYROSINE KINASES TO ACTIVATED GROWTH-FACTOR RECEPTORS [J].
ALONSO, G ;
KOEGL, M ;
MAZURENKO, N ;
COURTNEIDGE, SA .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (17) :9840-9848
[2]  
[Anonymous], STAT COMPUT
[3]  
[Anonymous], 2002, PROTEIN PROTEIN INTE
[4]  
Baccarini M, 1991, Receptor, V1, P243
[5]  
BACCARINI M, 1991, J BIOL CHEM, V266, P10941
[6]  
BOOCOCK CA, 1989, J CELL SCI, V93, P447
[7]   Early events in M-CSF receptor signaling [J].
Bourette, RP ;
Rohrschneider, LR .
GROWTH FACTORS, 2000, 17 (03) :155-166
[8]  
BOURETTE RP, 1995, CELL GROWTH DIFFER, V6, P631
[9]   Sequential activation of phoshatidylinositol 3-kinase and phospholipase C-gamma 2 by the M-CSF receptor is necessary for differentiation signaling [J].
Bourette, RP ;
Myles, GM ;
Choi, JL ;
Rohrschneider, LR .
EMBO JOURNAL, 1997, 16 (19) :5880-5893
[10]   Expression of Mona (monocytic adapter) in myeloid progenitor cells results in increased and prolonged MAP kinase activation upon macrophage colony-stimulating factor stimulation [J].
Bourgin, C ;
Bourette, R ;
Mouchiroud, G ;
Arnaud, S .
FEBS LETTERS, 2000, 480 (2-3) :113-117