Morphological interactions of human first trimester placental villi co-cultured with decidual explants

被引:23
作者
Babawale, MO
VanNoorden, S
Pignatelli, M
Stamp, GWH
Elder, MG
Sullivan, MHF
机构
[1] HAMMERSMITH HOSP, ROYAL POSTGRAD MED SCH, INST OBSTET & GYNAECOL, LONDON W12 0NN, ENGLAND
[2] HAMMERSMITH HOSP, ROYAL POSTGRAD MED SCH, DEPT HISTOPATHOL, LONDON W12 0NN, ENGLAND
关键词
decidua; E-cadherin; Ki-67; placentation; trophoblast;
D O I
10.1093/HUMREP/11.2.444
中图分类号
R71 [妇产科学];
学科分类号
100211 ;
摘要
Abnormalities of pregnancy such as pre-eclampsia and intrauterine growth retardation are characterized by shallow trophoblastic invasion of the placental bed, the precise molecular pathophysiology of which remains to be fully elucidated. An in-vitro model involving a co-culture of first trimester placental villi and decidua parietalis explants (of 8-12 weeks gestation) was developed and used to characterize the migration and local invasion of trophoblast cells, Trophoblast proliferation (confirmed by Ki-67 immunostaining), differentiation and loose attachment of placental villi to the underlying decidual epithelium or stroma occurred within the first 24 h of co-culture. This was followed by erosion of the syncytial layer of the placental villi and commencement of a progressive cytotrophoblast invasion after 48 h of co-culture, which continued until 120 h, when the experiments were terminated. E-cadherin was expressed at the interfaces between trophoblast cells within the villi, but expression of this adhesion molecule seemed to be down-regulated in the invasive trophoblast cells. Our results suggest that the model could be useful in investigating the factors that control early human placentation and the fete-maternal interface.
引用
收藏
页码:444 / 450
页数:7
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