Platelet activating factor receptor expression is associated with neuronal apoptosis in an in vivo model of excitotoxicity

被引:33
作者
Bennett, SAL
Chen, JH
Pappas, BA
Roberts, DCS
Tenniswood, M
机构
[1] Carleton Univ, Inst Biochem, Life Sci Res Ctr, Ottawa, ON K1S 5B6, Canada
[2] Carleton Univ, Inst Neurosci, Ottawa, ON K1S 5B6, Canada
[3] Adirondack Biomed Res Inst, Lake Placid, NY 12946 USA
关键词
platelet activating factor; apoptosis; neurodegeneration; kainic acid; epilepsy; excitotoxicity;
D O I
10.1038/sj.cdd.4400434
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Platelet activating factor (PAF), an endogenous proinflammatory agent, mediates neuronal survival, glutamate release, and transcriptional activation following excitotoxin challenge. To determine whether PAF receptor (PAFR) expression is altered during excitotoxicity, changes in PAFR mRNA localization were compared with markers of neuronal apoptosis and reactive gliosis following systemic injection of kainic acid. Data from semi-quantitative RT-PCR, in situ hybridization, DNA fragmentation, cellular morphology analysis, and immunohistochemistry demonstrate that the localization of PAFR mRNA is altered during kainic acid-induced neurodegeneration. While PAFR mRNA is normally exhibited by neurons and microglia in rat hippocampus, expression becomes restricted to apoptotic neurons and to glia involved in phagocytosing apoptotic debris following treatment with excitotoxin. PAFR mRNA is rarely detected in surviving neurons. These data provide the first indication that PAFR-expressing neurons may be preferentially susceptible to excitotoxic challenge.
引用
收藏
页码:867 / 875
页数:9
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