A novel secreted tumor antigen with a glycosylphosphatidylinositol-anchored structure ubiquitously expressed in human cancers

被引:26
作者
Onda, H
Ohkubo, S
Shintani, Y
Ogi, K
Kikuchi, K
Tanaka, H
Yamamoto, K
Tsuji, I
Ishibashi, Y
Yamada, T
Kitada, C
Suzuki, N
Sawada, H
Nishimura, O
Fujino, M
机构
[1] Takeda Chem Ind Ltd, Div Pharmaceut Res, Discovery Res Labs 1, Tsukuba, Ibaraki 3004293, Japan
[2] Takeda Chem Ind Ltd, Div Pharmaceut Res, Discovery Res Labs 2, Tsukuba, Ibaraki 3004293, Japan
[3] Takeda Chem Ind Ltd, Div Pharmaceut Res, Pharmaceut Discovery Ctr, Yodogawa Ku, Osaka 5328686, Japan
关键词
ALCAN; tumor-specific gene; GPI-anchored protein; tumor antigen; tumor marker;
D O I
10.1006/bbrc.2001.5149
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In a search for novel genes expressed in human cancers, we identified one gene from an assembled expressed sequence tag database. Northern blot analysis revealed that the gene, termed alcan, was expressed in various types of human cancer cell lines and in the fetus, but not in normal tissues. The alcan gene is located on chromosome 6 and is encoded on a 246-amino-acid protein with weak homology to classical major histocompatibility complex class I. Its gene product, ALCAN, had hydrophobic amino acid clusters at both the N- and C-terminal regions and was predicted to be a glycosylphosphatidylinositol (GPI)anchored membrane protein. Flow cytometric analysis revealed that ALCAN was detected on the surface of human cancer cells and on alcan-transfected CHO-K1 cells. ALCAN was also secreted from these cells, suggesting that some portion of the molecules was secreted by enzymatic cleavage by, for example, phospholipases. Mutational analysis of ALCAN suggested that the GPI-anchored position was the Ser(216) residue. These findings indicate that ALCAN may be a potential target for cancer diagnosis or therapy, (C) 2001 Academic Press.
引用
收藏
页码:235 / 243
页数:9
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