The 25 degrees-36 degrees region of the Bacillus subtilis chromosome: Determination of the sequence of a 146 kb segment and identification of 113 genes

被引：28

作者：

Yamane, K

Kumano, M

Kurita, K

机构：

[1] Institute of Biological Sciences, University of Tsukuba, Tsukuba-shi

来源：

MICROBIOLOGY-SGM | 1996年 / 142卷

关键词：

Bacillus subtilis; genome sequencing; amyE-srfA region;

D O I：

10.1099/13500872-142-11-3047

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We determined a 146 kb contiguous sequence at the 25 degrees-36 degrees region of the Bacillus subtilis chromosome containing the amyE-srfA segment. Among the 113 ORFs identified, 33 are already known. Functions were assigned to 38 ORFs by a search of non-redundant protein sequence data banks and those of 16 ORFs were suggested through significant similarity with reported sequences. The amino acid sequences of 13 of the ORFs were similar to proteins of unknown function of Escherichia coli, Haemophilus influenzae and other species. We did not find similarities for 29 ORFs to any known proteins. The 146 kb region is rich in enzymes (35 ORFs) related to the metabolism of low molecular mass compounds and five genes for surfactin production occupy about 26 kb of the region.

引用

页码：3047 / 3056

页数：10

共 39 条

[1] Determination of a 17484 bp nucleotide sequence around the 39 degrees region of the Bacillus subtilis chromosome and similarity analysis of the products of putative ORFs [J].

Akagawa, E ;

Kurita, K ;

Sugawara, T ;

Nakamura, K ;

Kasahara, Y ;

Ogasawara, N ;

Yamane, K .

MICROBIOLOGY-UK, 1995, 141 :3241-3245

[2] NUCLEOTIDE-SEQUENCE OF THE OUTB LOCUS OF BACILLUS-SUBTILIS AND REGULATION OF ITS EXPRESSION [J].

ALBERTINI, AM ;

CARAMORI, T ;

HENNER, D ;

FERRARI, E ;

GALIZZI, A .

JOURNAL OF BACTERIOLOGY, 1987, 169 (04) :1480-1484

[3]

Anagnostopoulos C., 1993, P425

[4] SEQUENCE AND ANALYSIS OF THE GENETIC-LOCUS RESPONSIBLE FOR SURFACTIN SYNTHESIS IN BACILLUS-SUBTILIS [J].

COSMINA, P ;

RODRIGUEZ, F ;

DEFERRA, F ;

GRANDI, G ;

PEREGO, M ;

VENEMA, G ;

VANSINDEREN, D .

MOLECULAR MICROBIOLOGY, 1993, 8 (05) :821-831

[5] IDENTIFICATION OF COMS, A GENE OF THE SRFA OPERON THAT REGULATES THE ESTABLISHMENT OF GENETIC COMPETENCE IN BACILLUS-SUBTILIS [J].

DSOUZA, C ;

NAKANO, MM ;

ZUBER, P .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (20) :9397-9401

[6] COMPLETE DNA-SEQUENCE OF YEAST CHROMOSOME-XI [J].

DUJON, B ;

ALEXANDRAKI, D ;

ANDRE, B ;

ANSORGE, W ;

BALADRON, V ;

BALLESTA, JPG ;

BANREVI, A ;

BOLLE, PA ;

BOLOTINFUKUHARA, M ;

BOSSIER, P ;

BOU, G ;

BOYER, J ;

BUITRAGO, MJ ;

CHERET, G ;

COLLEAUX, L ;

DALGNANFORNIER, B ;

DELREY, F ;

DION, C ;

DOMDEY, H ;

DUSTERHOFT, A ;

DUSTERHUS, S ;

ENTLAN, KD ;

ERFLE, H ;

ESTEBAN, PF ;

FELDMANN, H ;

FERNANDES, L ;

FOBO, GM ;

FRITZ, C ;

FUKUHARA, H ;

GABEL, C ;

GAILLON, L ;

CARCIACANTALEJO, JM ;

GARCIARAMIREZ, JJ ;

GENT, ME ;

GHAZVINI, M ;

GOFFEAU, A ;

GONZALEZ, A ;

GROTHUES, D ;

GUERREIRO, P ;

HEGEMANN, J ;

HEWITT, N ;

HILGER, F ;

HOLLENBERG, CP ;

HORAITIS, O ;

INDGE, KJ ;

JACQUIER, A ;

JAMES, CM ;

JAUNIAUX, JC ;

JIMENEZ, A ;

KEUCHEL, H .

NATURE, 1994, 369 (6479) :371-378

[7] WHOLE-GENOME RANDOM SEQUENCING AND ASSEMBLY OF HAEMOPHILUS-INFLUENZAE RD [J].

FLEISCHMANN, RD ;

ADAMS, MD ;

WHITE, O ;

CLAYTON, RA ;

KIRKNESS, EF ;

KERLAVAGE, AR ;

BULT, CJ ;

TOMB, JF ;

DOUGHERTY, BA ;

MERRICK, JM ;

MCKENNEY, K ;

SUTTON, G ;

FITZHUGH, W ;

FIELDS, C ;

GOCAYNE, JD ;

SCOTT, J ;

SHIRLEY, R ;

LIU, LI ;

GLODEK, A ;

KELLEY, JM ;

WEIDMAN, JF ;

PHILLIPS, CA ;

SPRIGGS, T ;

HEDBLOM, E ;

COTTON, MD ;

UTTERBACK, TR ;

HANNA, MC ;

NGUYEN, DT ;

SAUDEK, DM ;

BRANDON, RC ;

FINE, LD ;

FRITCHMAN, JL ;

FUHRMANN, JL ;

GEOGHAGEN, NSM ;

GNEHM, CL ;

MCDONALD, LA ;

SMALL, KV ;

FRASER, CM ;

SMITH, HO ;

VENTER, JC .

SCIENCE, 1995, 269 (5223) :496-512

[8] A 10-KB NUCLEOTIDE-SEQUENCE AT THE 5' FLANKING REGION (32-DEGREES) OF SRFAA OF THE BACILLUS-SUBTILIS CHROMOSOME [J].

FUJISHIMA, Y ;

YAMANE, K .

MICROBIOLOGY-UK, 1995, 141 :277-279

[9] NUCLEOTIDE-SEQUENCE OF 5' PORTION OF SRFA THAT CONTAINS THE REGION REQUIRED FOR COMPETENCE ESTABLISHMENT IN BACILLUS-SUBTILIS [J].

FUMA, S ;

FUJISHIMA, Y ;

CORBELL, N ;

DSOUZA, C ;

NAKANO, MM ;

ZUBER, P ;

YAMANE, K .

NUCLEIC ACIDS RESEARCH, 1993, 21 (01) :93-97

[10] IDENTIFICATION OF TLPC, A NOVEL 62-KDA MCP-LIKE PROTEIN FROM BACILLUS-SUBTILIS [J].

HANLON, DW ;

ROSARIO, MML ;

ORDAL, GW ;

VENEMA, G ;

VANSINDEREN, D .

MICROBIOLOGY-SGM, 1994, 140 :1847-1854

← 1 2 3 4 →