Heme oxygenase-1 induction by endogenous nitric oxide:: influence of intracellular glutathione

被引：29

作者：

André, M ^{[1
]}

Felley-Bosco, E ^{[1
]}

机构：

[1] Univ Lausanne, Inst Pharmacol & Toxicol, CH-1005 Lausanne, Switzerland

来源：

FEBS LETTERS | 2003年 / 546卷 / 2-3期

关键词：

heme oxygenase-1; nitric oxide; glutathione; oxidative stress;

D O I：

10.1016/S0014-5793(03)00576-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To investigate the influence of glutathione (GSH) on cellular effects of nitric oxide (NO) formation, human colon adenocarcinoma cells were transfected with a vector allowing controlled expression of inducible nitric oxide synthase (iNOS). Protein levels of oxidative stree-sensitive heme oxygenase-1 (HO-1) were analyzed in the presence or absence of GSH depletion using (L)-buthionine-[S,R]-sulfoximine and iNOS induction. While no effect was observed in the presence of iNOS activity alone, a synergic effect on HO-1 expression was observed in the presence of iNOS expression and GSH depletion. This effect eas prevented by addition of N-methyl-(L)-arginine. Therefore, targeting of endogenous NO may be modulated by intracellular GSH. (C) 2003 Pulblished by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.

引用

页码：223 / 227

页数：5

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