Influence of residue 44 on the activity of the M2 proton channel of influenza A virus

被引:38
作者
Betakova, T
Ciampor, F
Hay, AJ
机构
[1] Slovak Acad Sci, Inst Virol, Bratislava 84505, Slovakia
[2] Natl Inst Med Res, London NW7 1AA, England
关键词
D O I
10.1099/vir.0.80358-0
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The influenza A virus M2 proton channel plays a role in two stages of virus replication. The proteins of two closely related strains of the avian H7 subtype of influenza A virus, Rostock and Weybridge, were found to differ in their pH-modulating activities and activation characteristics. Of three amino acid differences at residues 27, 38 and 44 within the membrane-spanning domain, substitution at residue 44 was necessary and sufficient to account for differences in trans-Golgi pH-modulating activity, whereas changes in all three were required to switch the activation characteristics of the Weybridge M2 to those of the Rostock M2. These results not only separate the two phenomena genetically, but also indicate that the 'unique' activation characteristics of the Rostock M2 channel were selected specifically. In addition, they point to the importance of functional complementarity between the activation characteristics of the M2 channel and the pH of membrane fusion by haemagglutinin during virus entry.
引用
收藏
页码:181 / 184
页数:4
相关论文
共 12 条
[1]   Differences in conductance of M2 proton channels of two influenza viruses at low and high pH [J].
Chizhmakov, IV ;
Ogden, DC ;
Geraghty, FM ;
Hayhurst, A ;
Skinner, A ;
Betakova, T ;
Hay, AJ .
JOURNAL OF PHYSIOLOGY-LONDON, 2003, 546 (02) :427-438
[2]   Selective proton permeability and pH regulation of the influenza virus M2 channel expressed in mouse erythroleukaemia cells [J].
Chizhmakov, IV ;
Geraghty, FM ;
Ogden, DC ;
Hayhurst, A ;
Antoniou, M ;
Hay, AJ .
JOURNAL OF PHYSIOLOGY-LONDON, 1996, 494 (02) :329-336
[3]   EVIDENCE THAT THE AMANTADINE-INDUCED, M2-MEDIATED CONVERSION OF INFLUENZA-A VIRUS HEMAGGLUTININ TO THE LOW PH CONFORMATION OCCURS IN AN ACIDIC TRANSGOLGI COMPARTMENT [J].
CIAMPOR, F ;
BAYLEY, PM ;
NERMUT, MV ;
HIRST, EMA ;
SUGRUE, RJ ;
HAY, AJ .
VIROLOGY, 1992, 188 (01) :14-24
[4]   EUKARYOTIC TRANSIENT-EXPRESSION SYSTEM BASED ON RECOMBINANT VACCINIA VIRUS THAT SYNTHESIZES BACTERIOPHAGE-T7 RNA-POLYMERASE [J].
FUERST, TR ;
NILES, EG ;
STUDIER, FW ;
MOSS, B .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (21) :8122-8126
[5]   INFLUENCE OF AMANTADINE RESISTANCE MUTATIONS ON THE PH REGULATORY FUNCTION OF THE M2-PROTEIN OF INFLUENZA-A VIRUSES [J].
GRAMBAS, S ;
BENNETT, MS ;
HAY, AJ .
VIROLOGY, 1992, 191 (02) :541-549
[6]   MATURATION OF INFLUENZA-A VIRUS HEMAGGLUTININ - ESTIMATES OF THE PH ENCOUNTERED DURING TRANSPORT AND ITS REGULATION BY THE M2 PROTEIN [J].
GRAMBAS, S ;
HAY, AJ .
VIROLOGY, 1992, 190 (01) :11-18
[7]   NUCLEAR TRANSPORT OF INFLUENZA-VIRUS RIBONUCLEOPROTEINS - THE VIRAL MATRIX PROTEIN (M1) PROMOTES EXPORT AND INHIBITS IMPORT [J].
MARTIN, K ;
HELENIUS, A .
CELL, 1991, 67 (01) :117-130
[8]   Permeation and activation of the M2 ion channel of influenza A virus [J].
Mould, JA ;
Drury, JE ;
Frings, SM ;
Kaupp, UB ;
Pekosz, A ;
Lamb, RA ;
Pinto, LH .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (40) :31038-31050
[9]   INFLUENZA-VIRUS M2 PROTEIN HAS ION CHANNEL ACTIVITY [J].
PINTO, LH ;
HOLSINGER, LJ ;
LAMB, RA .
CELL, 1992, 69 (03) :517-528
[10]   AMANTADINE SELECTION OF A MUTANT INFLUENZA-VIRUS CONTAINING AN ACID-STABLE HEMAGGLUTININ GLYCOPROTEIN - EVIDENCE FOR VIRUS-SPECIFIC REGULATION OF THE PH OF GLYCOPROTEIN TRANSPORT VESICLES [J].
STEINHAUER, DA ;
WHARTON, SA ;
SKEHEL, JJ ;
WILEY, DC ;
HAY, AJ .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (24) :11525-11529