Relationship between DNA damage, total antioxidant capacity and coronary artery disease

Objective : It has been known that there was a relation between the levels of DNA damage and the severity of the coronary artery disease (CAD). However, little is known about association of DNA damage with total antioxidant capacity (TAC) and CAD. The aim of this study is to evaluate the relationship between DNA damage, TAC and CAD. Methods : We used the comet assay to measure DNA damage from 53 patients with angiographically documented CAD and 42 patients with angiographically documented normal coronary vessel. The extent and severity of CAD was calculated to Gensini score index. TAC of plasma was determined using a novel automated measurement method. Results : Mean values of DNA damage were significantly higher in CAD patients than in the control group (p < 0.001). There was a positive correlation between Gensini score index and DNA damage (r = 0.590, p < 0.001). Additionally, significantly positive correlations between score of DNA damage, and diabetes, smoking, obesity and hyperlipidemia were found (p < 0.05). There was also a negative correlation between TAC and DNA damage (r = -0.711, p < 0.001). The DNA damage was significantly higher in diabetic, smoker, hyperlipidemic and obese individuals than those without these conditions (p = 0.001, p = 0.006, p = 0.001, p = 0.004, respectively). Conclusions : These data indicate that the level of DNA damage is increased and TAC level is decreased in CAD. DNA damage is correlated with the severity of the CAD, and levels of TAC. (C) 2004 Elsevier B.V. All rights reserved.