Detection of mutations in parC in quinolone-resistant clinical isolates of Escherichia coli

被引:190
作者
Vila, J
Ruiz, J
Goni, P
DeAnta, MTJ
机构
关键词
D O I
10.1128/AAC.40.2.491
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The gene parC encodes the A subunit of topoisomerase IV of Escherichia coli. Mutations in the parC region analogous to those in the quinolone resistance-determining region of gyrA were investigated in 27 clinical isolates of E. coli for which ciprofloxacin MICs were 0.007 to 128 mu g/ml. Of 15 isolates for which ciprofloxacin MICs were greater than or equal to 1 mu g/ml, 8 showed a change in the serine residue at position 80 (Ser-80), 4 showed a change in Glu-84, and 3 showed changes in both amino acids. No mutations were detected in 12 clinical isolates for which ciprofloxacin MICs were less than or equal to 0.25 mu g/ml. These findings suggest that ParC from E. coli may be another target for quinolones and that mutations at residues Ser-80 and Glu-84 may contribute to decreased fluoroquinolone susceptibility.
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页码:491 / 493
页数:3
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