Cytoplasmic Partitioning of P Granule Components Is Not Required to Specify the Germline in C. elegans

被引：110

作者：

Gallo, Christopher M. ^{[1
]}

Wang, Jennifer T. ^{[1
]}

Motegi, Fumio ^{[1
]}

Seydoux, Geraldine ^{[1
]}

机构：

[1] Johns Hopkins Sch Med, Ctr Cell Dynam, Howard Hughes Med Inst, Dept Mol Biol & Genet, Baltimore, MD 21205 USA

来源：

SCIENCE | 2010年 / 330卷 / 6011期

关键词：

CAENORHABDITIS-ELEGANS; ASYMMETRY; PROTEINS;

D O I：

10.1126/science.1193697

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

070301 [无机化学]; 070403 [天体物理学]; 070507 [自然资源与国土空间规划学]; 090105 [作物生产系统与生态工程];

摘要：

Asymmetric segregation of P granules during the first four divisions of the Caenorhabditis elegans embryo is a classic example of cytoplasmic partitioning of germline determinants. It is thought that asymmetric partitioning of P granule components during mitosis is essential to distinguish germline from soma. We have identified a mutant (pptr-1) in which P granules become unstable during mitosis and P granule proteins and RNAs are distributed equally to somatic and germline blastomeres. Despite symmetric partitioning of P granule components, pptr-1 mutants segregate a germline that uniquely expresses P granules during postembryonic development. pptr-1 mutants are fertile, except at high temperatures. Hence, asymmetric partitioning of maternal P granules is not essential to specify germ cell fate. Instead, it may serve to protect the nascent germline from stress.

引用

页码：1685 / 1689

页数：5

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