Real-time PCR assay targets the 23S-5S spacer for direct detection and differentiation of Legionella spp. and Legionella pneumophila

被引：67

作者：

Herpers, BL

de Jongh, BM

van der Zwaluw, K

van Hannen, EJ

机构：

[1] St Antonius Hosp, Dept Immunol & Med Microbiol, NL-3435 CM Nieuwegein, Netherlands

[2] Natl Inst Publ Hlth & Environm, LIS, NL-3720 BA Bilthoven, Netherlands

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2003年 / 41卷 / 10期

关键词：

D O I：

10.1128/JCM.41.10.4815-4816.2003

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A real-time PCR for the ABI Prism 7000 system targeting the 23S-5S spacer of Legionella spp. was developed. Simultaneous detection and differentiation of Legionella spp. and Legionella pneumophild within 90 min and without post-PCR melting-curve analysis was achieved using two TaqMan probes. In sputum samples from 23 controls and 17 patients with legionellosis, defined by positive culture, urinary antigen testing, or seroconversion, 94% sensitivity and 100% specificity were observed.

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页码：4815 / 4816

页数：2

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