Varicella-zoster virus ORF57, unlike its pseudorabies virus UL3.5 homolog, is dispensable for viral replication in cell culture

被引：24

作者：

Cox, E ^{[1
]}

Reddy, S ^{[1
]}

Iofin, I ^{[1
]}

Cohen, JI ^{[1
]}

机构：

[1] NIAID, Med Virol Sect, Clin Invest Lab, NIH, Bethesda, MD 20892 USA

来源：

VIROLOGY | 1998年 / 250卷 / 01期

关键词：

D O I：

10.1006/viro.1998.9349

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Varicella tester virus (VZV) encodes five genes that do not have homologs in herpes simplex virus. One of these genes, VN ORF57, is predicted to encode a protein containing 71 amino acids. Antibody to ORF57 protein immunoprecipitated a 6-kDa protein in the cytosol of VZV-infected cells. Although the homolog of VZV ORF57 in pseudorabies virus, UL3.5, is critical for viral egress and growth in cell culture, VN unable to express ORF57 replicated to titers similar to those seen with parental virus. Thus VZV ORF57 has a different role in Viral replication than its pseudorabies virus homolog.

引用

页码：205 / 209

页数：5

共 13 条

[1]

COHEN IL, 1996, FIELDS VIROLOGY, P2525

[2] VARICELLA-ZOSTER VIRUS OPEN READING FRAME-1 ENCODES A MEMBRANE-PROTEIN THAT IS DISPENSABLE FOR GROWTH OF VZV IN-VITRO [J].

COHEN, JI ;

SEIDEL, KE .

VIROLOGY, 1995, 206 (02) :835-842

[3] GENERATION OF VARICELLA-ZOSTER VIRUS (VZV) AND VIRAL MUTANTS FROM COSMID DNAS - VZV THYMIDYLATE SYNTHETASE IS NOT ESSENTIAL FOR REPLICATION IN-VITRO [J].

COHEN, JI ;

SEIDEL, KE .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (15) :7376-7380

[4] THE COMPLETE DNA-SEQUENCE OF VARICELLA-ZOSTER VIRUS [J].

DAVISON, AJ ;

SCOTT, JE .

JOURNAL OF GENERAL VIROLOGY, 1986, 67 :1759-1816

[5] A 3' COTERMINAL GENE-CLUSTER IN PSEUDORABIES VIRUS CONTAINS HERPES-SIMPLEX VIRUS UL1, UL2, AND UL3 GENE HOMOLOGS AND A UNIQUE UL3.5 OPEN READING FRAME [J].

DEAN, HJ ;

CHEUNG, AK .

JOURNAL OF VIROLOGY, 1993, 67 (10) :5955-5961

[6] DNA sequence and transcriptional analysis of the UL1 to UL5 gene cluster of infectious laryngotracheitis virus [J].

Fuchs, W ;

Mettenleiter, TC .

JOURNAL OF GENERAL VIROLOGY, 1996, 77 :2221-2229

[7] Identification and characterization of the pseudorabies virus UL3.5 protein, which is involved in virus egress [J].

Fuchs, W ;

Klupp, BG ;

Granzow, H ;

Rziha, HJ ;

Mettenleiter, TC .

JOURNAL OF VIROLOGY, 1996, 70 (06) :3517-3527

[8] Functional complementation of UL3.5-negative pseudorabies virus by the bovine herpesvirus 1 UL3.5 homolog [J].

Fuchs, W ;

Granzow, H ;

Mettenleiter, TC .

JOURNAL OF VIROLOGY, 1997, 71 (11) :8886-8892

[9] IDENTIFICATION AND TRANSCRIPTIONAL ANALYSIS OF A 3'-COTERMINAL GENE-CLUSTER CONTAINING UL1, UL2, UL3, AND UL3.5 OPEN READING FRAMES OF BOVINE HERPESVIRUS-1 [J].

KHATTAR, SK ;

LITTELVANDENHURK, SV ;

BABIUK, LA ;

TIKOO, SK .

VIROLOGY, 1995, 213 (01) :28-37

[10]

REDDY SM, 1998, IN PRESS J VIROL

← 1 2 →