Ozone-induced inactivation of antioxidant enzymes

被引:18
作者
Lee, YK [1 ]
Kim, SM [1 ]
Han, S [1 ]
机构
[1] Kangwon Natl Univ, Coll Nat Sci, Dept Biochem, Chunchon 200701, South Korea
关键词
ozone; superoxide dismutase; catalase; glutathione peroxidase; antioxidants;
D O I
10.1016/j.biochi.2003.09.012
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Ozone is an air pollutant that damages a variety of biomolecules. We investigated ozone-induced inactivation of three major antioxidant enzymes. Cu/Zn superoxide dismutase was inactivated by ozone in a concentration-dependent manner. The concentration of ozone for 50% inactivation was similar to45 muM when 10 muM Cu/Zn superoxide dismutase was incubated for 30 min in the presence of ozone. SDS-polyacrylamide gel electrophoresis (PAGE) showed that the enzyme was randomly fragmented. Both ascorbate and glutathione were very effective in protecting Cu/Zn superoxide dismutase from ozone-induced inactivation. The other two enzymes, catalase and glutathione peroxidase, were much more resistant to ozone than Cu/Zn superoxide dismutase. The ozone concentrations for 50% inactivation of 10 muM catalase and glutathione peroxidase were 500 and 240 muM, respectively. SDS-PAGE demonstrated that ozone caused formation of high molecular weight aggregates in catalase and dimerization in glutathione peroxidase. Glutathione protected catalase and glutathione peroxidase from ozone but the effective concentrations were much higher than that for Cu/Zn superoxide dismutase. Ascorbate was almost ineffective. The result suggests that, among the three antioxidant enzymes, Cu/Zn superoxide dismutase is a major target for ozone-induced inactivation and both glutathione and ascorbate are very effective in protecting the enzyme from ozone. (C) 2003 Editions scientifiques et medicales Elsevier SAS. All rights reserved.
引用
收藏
页码:947 / 952
页数:6
相关论文
共 28 条
[1]   Investigating antibody-catalyzed ozone generation by human neutrophils [J].
Babior, BM ;
Takeuchi, C ;
Ruedi, J ;
Gutierrez, A ;
Wentworth, P .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (06) :3031-3034
[2]   Comparison of the effects of ozone on the modification of amino acid residues in glutamine synthetase and bovine serum albumin [J].
Berlett, BS ;
Levine, RL ;
Stadtman, ER .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (08) :4177-4182
[3]  
Bilgin R, 1999, BIOCHEM MOL BIOL INT, V47, P227
[4]  
BROWN LAS, 1996, ANTIOX HEALTH DIS, V3, P117
[5]   DETECTION OF HYDROXYL RADICALS UPON INTERACTION OF OZONE WITH AQUEOUS-MEDIA OR EXTRACELLULAR SURFACTANT - THE ROLE OF TRACE IRON [J].
BYVOET, P ;
BALIS, JU ;
SHELLEY, SA ;
MONTGOMERY, MR ;
BARBER, MJ .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1995, 319 (02) :464-469
[6]   TISSUE TRACE-ELEMENTS AND LUNG SUPEROXIDE-DISMUTASE ACTIVITY IN MICE EXPOSED TO OZONE [J].
DUBICK, MA ;
KEEN, CL .
TOXICOLOGY LETTERS, 1983, 17 (3-4) :355-360
[7]   Influence of catalase and superoxide dismutase on ozone inactivation of Listeria monocytogenes [J].
Fisher, CW ;
Lee, D ;
Dodge, BA ;
Hamman, KM ;
Robbins, JB ;
Martin, SE .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2000, 66 (04) :1405-1409
[8]   Evaluation of 8-oxodeoxyguanosine, typical oxidative DNA damage, in lymphocytes of ozone-treated arteriosclerotic patients [J].
Foksinski, M ;
Bialkowski, K ;
Skiba, M ;
Ponikowska, I ;
Szmurlo, W ;
Olinski, R .
MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS, 1999, 438 (01) :23-27
[9]   Aromatic hydroxylation in nasal lavage fluid following ambient ozone exposure [J].
Frischer, T ;
Pullwitt, A ;
Kuhr, J ;
Meinert, R ;
Haschke, N ;
Studnicka, M ;
Lubec, G .
FREE RADICAL BIOLOGY AND MEDICINE, 1997, 22 (1-2) :201-207
[10]   Early detection of ozone-induced hydroperoxides in epithelial cells by a novel infrared spectroscopic method [J].
Hemmingsen, A ;
Allen, JT ;
Zhang, SF ;
Mortensen, J ;
Spiteri, MA .
FREE RADICAL RESEARCH, 1999, 31 (05) :437-448