Metabolic labeling of fucosylated glycoproteins in Bacteroidales species

被引：43

作者：

Besanceney-Webler, Christen ^{[2
]}

Jiang, Hao ^{[2
]}

Wang, Wei ^{[2
]}

Baughn, Anthony D. ^{[1
]}

Wu, Peng ^{[2
]}

机构：

[1] Univ Minnesota, Dept Microbiol, Minneapolis, MN 55455 USA

[2] Yeshiva Univ, Albert Einstein Coll Med, Dept Biochem, Bronx, NY 10461 USA

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2011年 / 21卷 / 17期

基金：

美国国家卫生研究院;

关键词：

Click chemistry; Fucosylated glycans; Metabolic engineering; IN-VIVO; COMMENSAL; MICROBES; BACTERIA; GLYCANS; SURFACE; PHASE;

D O I：

10.1016/j.bmcl.2011.05.038

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Members of the Bacteroidales order are among the most abundant gram-negative bacteria of the human colonic microbiota. These species decorate their cell-surface glycoproteins with fucosylated glycans, which are believed to play important roles in host intestinal colonization. Currently, there is no method for the enrichment of these glycoproteins for their identification. Here, we describe a chemical approach directed toward labeling and detecting fucosylated glycoproteins from cultured Bacteroidales species, namely Bacteroides fragilis and Parabacteroides distasonis. We treated these bacteria with an alkyne-bearing fucose analog, which is metabolically integrated into the bacterial surface fucosylated glycoproteins. The alkyne-tagged glycoproteins can then react with azide-bearing biophysical probes via bioorthogonal click chemistry for detection or glycoproteomic analysis. (C) 2011 Elsevier Ltd. All rights reserved.

引用

页码：4989 / 4992

页数：4

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