The dog and rat olfactory receptor repertoires

被引:101
作者
Quignon, P
Giraud, M
Rimbault, M
Lavigne, P
Tacher, S
Morin, E
Retout, E
Valin, AS
Lindblad-Toh, K
Nicolas, J
Galibert, F [1 ]
机构
[1] Univ Rennes 1, Genet & Dev CNRS, UMR 6061, F-35043 Rennes, France
[2] IRISA, F-35042 Rennes, France
[3] MIT, Broad Inst, Cambridge, MA 02141 USA
[4] Harvard Univ, Cambridge, MA 02141 USA
[5] NHGRI, NIH, Bethesda, MD 20892 USA
关键词
D O I
10.1186/gb-2005-6-10-r83
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Background: Dogs and rats have a highly developed capability to detect and identify odorant molecules, even at minute concentrations. Previous analyses have shown that the olfactory receptors (ORs) that specifically bind odorant molecules are encoded by the largest gene family sequenced in mammals so far. Results: We identified five amino acid patterns characteristic of ORs in the recently sequenced boxer dog and brown Norway rat genomes. Using these patterns, we retrieved 1,094 dog genes and 1,493 rat genes from these shotgun sequences. The retrieved sequences constitute the olfactory receptor repertoires of these two animals. Subsets of 20.3% ( for the dog) and 19.5% ( for the rat) of these genes were annotated as pseudogenes as they had one or several mutations interrupting their open reading frames. We performed phylogenetic studies and organized these two repertoires into classes, families and subfamilies. Conclusion: We have established a complete or almost complete list of OR genes in the dog and the rat and have compared the sequences of these genes within and between the two species. Our results provide insight into the evolutionary development of these genes and the local amplifications that have led to the specific amplification of many subfamilies. We have also compared the human and rat ORs with the human and mouse OR repertoires.
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