Dynamic repositioning of genes in the nucleus of lymphocytes preparing for cell division

被引：351

作者：

Brown, KE ^{[1
]}

Baxter, J ^{[1
]}

Graf, D ^{[1
]}

Merkenschlager, M ^{[1
]}

Fisher, AG ^{[1
]}

机构：

[1] Hammersmith Hosp, Imperial Coll Sch Med, MRC Clin Sci Ctr, Lymphocyte Dev Grp, London W12 0NN, England

来源：

MOLECULAR CELL | 1999年 / 3卷 / 02期

基金：

英国医学研究理事会;

关键词：

D O I：

10.1016/S1097-2765(00)80311-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We show that several transcriptionally inactive genes localize to centromeric heterochromatin in the nucleus of cycling but not quiescent (noncycling) primary B lymphocytes. In quiescent cells, centromeric repositioning of inactive loci was induced after mitogenic stimulation. A dynamic repositioning of selected genes was also observed in developing T cells. Rag and TdT loci were shown to relocate to centromeric domains following heritable gene silencing in primary CD4(+)8(+) thymocytes, but not in a phenotypically similar cell line in which silencing occurred but was not heritable. Collectively, these data indicate that the spatial organization of genes in cycling and noncycling lymphocytes is different and that locus repositioning may be a feature of heritable gene silencing.

引用

页码：207 / 217

页数：11

共 69 条

[1] The role of mel-18, a mammalian Polycomb group gene, during IL-7-dependent proliferation of lymphocyte precursors [J].