Biotinylation of histones in human cells - Effects of cell proliferation

被引：130

作者：

Stanley, JS

Griffin, JB

Zempleni, J

机构：

[1] Univ Nebraska, Dept Nutr Sci & Dietet, Lincoln, NE 68583 USA

[2] Univ Arkansas Med Sci, Dept Pediat, Div Immunol & Allergy, Little Rock, AR 72205 USA

[3] Arkansas Childrens Hosp, Res Inst, Little Rock, AR 72202 USA

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 2001年 / 268卷 / 20期

关键词：

biotin; biotinidase; cell proliferation; histones;

D O I：

10.1046/j.0014-2956.2001.02481.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An enzymatic mechanism has been proposed by which biotinidase may catalyze biotinylation of histones. Here, human cells were found to covalently bind biotin to histones H1, H2A, H2B, H3, and H4. Cells respond to proliferation with increased biotinylation of histones; biotinylation increases early in the cell cycle and remains increased during the cycle. Notwithstanding the catalytic role of biotinidase in biotinylation of histones, mRNA encoding biotinidase and biotinidase activity did not parallel the increased biotinylation of histones in proliferating cells. Biotinylation of histones might be regulated by enzymes other than biotinidase or by the rate of histone debiotinylation.

引用

页码：5424 / 5429

页数：6

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