Mass spectrometric method for analyzing metabolites in yeast with single cell sensitivity

被引：112

作者：

Amantonico, Andrea ^{[1
]}

Oh, Joo Yeon ^{[1
]}

Sobek, Jens ^{[2
]}

Heinemann, Matthias ^{[3
]}

Zenobi, Renato ^{[1
]}

机构：

[1] ETH, Dept Chem & Appl Biosci, CH-8093 Zurich, Switzerland

[2] UZH ETH Zurich, Funct Genom Ctr Zurich, CH-8057 Zurich, Switzerland

[3] ETH, Inst Mol Syst Biol, CH-8093 Zurich, Switzerland

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2008年 / 47卷 / 29期

关键词：

high-throughput screening; mass spectrometry; metabolites; microscale preparation; single-cell analysis;

D O I：

10.1002/anie.200705923

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Getting a look-in: An optimized MALDI-MS procedure has been developed to detect endogenous primary metabolites directly in the cell extract. A detection limit corresponding to metabolites from less than a single cell has been attained, opening the door to single-cell metabolomics by mass spectrometry. (Figure Presented) © 2008 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：5382 / 5385

页数：4

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