Protective effects of Puerariae flos against ethanol-induced apoptosis on human neuroblastoma cell line SK-N-MC

被引：11

作者：

Jang, MH

Shin, MC

Kim, YJ

Chung, JH

Yim, SV

Kim, EH

Kim, Y

Kim, CJ ^{[1
]}

机构：

[1] Kyung Hee Univ, Coll Med, Dept Physiol, Dongdaemun Gu, Seoul 130701, South Korea

[2] Kyung Hee Univ, Coll Med, Dept Pharmacol, Dongdaemun Gu, Seoul 130701, South Korea

[3] Kangwon Natl Univ, Coll Med, Dept Pharmacol, Chunchon 200701, South Korea

[4] Semyung Univ, Coll Oriental Med, Dept Meridianol, Jechon 390711, Chungbuk, South Korea

[5] Semyung Univ, Coll Oriental Med, Dept Biochem, Jechon 390711, Chungbuk, South Korea

来源：

JAPANESE JOURNAL OF PHARMACOLOGY | 2001年 / 87卷 / 04期

关键词：

Puerariae flos; ethanol; apoptosis;

D O I：

10.1254/jjp.87.338

中图分类号：

R9 [药学];

学科分类号：

1007 [药学];

摘要：

Puerariae flos (PF) is an oriental medical herb for alcohol abuse. To investigate whether PF possesses protective effects against ethanol (EtOH)-induced cytotoxicity in the central nervous system, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, flow cytometric analysis, DNA fragmentation assay, and reverse transcription-polymerase chain reaction were performed on SK-N-MC human neuroblastoma cells. Cells treated with EtOH exhibited several apoptotic features, while those pre-treated with PF prior to EtOH exposure showed a decreased occurrence of apoptotic features. In addition, PF pre-treatment inhibited the EtOH-induced increase in caspase-3 mRNA expression. These results suggest that PF may exert protective effects against EtOH-induced apoptosis in human neuroblastoma cells.

引用

页码：338 / 342

页数：5

共 15 条

[1]

CHRONIC ETHANOL FEEDING INCREASES APOPTOSIS AND CELL-PROLIFERATION IN RAT-LIVER [J].