Development and validation of HPLC methods for the enantioselective analysis of bambuterol and albuterol

被引：30

作者：

Bartolincic, A

Druskovic, V

Sporec, A

Vinkovic, V

机构：

[1] Rudjer Boskovic Inst, HR-10002 Zagreb, Croatia

[2] Belupo Pharmaceut & Cosmet Ltd, R&D, Zagreb 10000, Croatia

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 2005年 / 36卷 / 05期

关键词：

chiral separation; antiasthmatic drugs; albuterol; bambuterol; chiral columns;

D O I：

10.1016/j.jpba.2004.09.006

中图分类号：

O65 [分析化学];

学科分类号：

070302 [分析化学]; 081704 [应用化学];

摘要：

Suitable HPLC methods for the direct separation of bambuterol and albuterol enantiomers were developed. The enantioseparation was tested on numerous commercial chiral HPLC columns. For bambuterol the most convenient separation was determined on amylose Chiralpak AD column, and for albuterol on vancomycine Chirobiotic V column. The mobile phase compositions were systematically studied to obtain, the optimal chromatographic methods. Validation of methods in selected conditions shows that the chosen methods are selective and precise with linear response of detector for both pairs of enantiomers. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：1003 / 1010

页数：8

共 25 条

[1]

DIRECT SEPARATION OF ALBUTEROL ENANTIOMERS IN BIOLOGICAL-FLUIDS AND PHARMACEUTICAL FORMULATIONS USING ALPHA(1)-ACID GLYCOPROTEIN AND PIRKLE UREA TYPE COLUMNS [J].