Prediction of relative retention times of triacylglycerols in non-aqueous reversed-phase high-performance liquid chromatography

被引:33
作者
Lin, JT
Snyder, LR
McKeon, TA
机构
[1] ARS, Western Reg Res Ctr, USDA, Albany, CA 94710 USA
[2] LC Resources Inc, Orinda, CA 94563 USA
关键词
relative retention times; retention prediction; triacylglycerols;
D O I
10.1016/S0021-9673(98)00134-4
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Accurate and simple methods for prediction of relative retention times of triacyglycerols in non-aqueous reversed-phase HPLC are presented here. The prediction is based on a simple calculation using the experimental relative retention time (min) of the triacylglycerol with the closest corresponding structure and taking into account the contributions (min) of functional groups present in the triacylglycerol for which the standard is not available to predict the relative retention time. The contribution of functional groups on the fatty acyl in the sn-2 position were greater than those at the sn-1(3) position, especially for polar functional groups such as the hydroxy group of ricinoleate, Another method of prediction which is based on the experimental relative retention time of tristearin and disregards the difference of sn-1(3) and sn-2 is also presented here. (C) 1998 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:43 / 49
页数:7
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