Molecular anatomy of α-glucosidase

被引：36

作者：

Kimura, A ^{[1
]}

机构：

[1] Hokkaido Univ, Grad Sch Agr, Div Appl Biosci, Sapporo, Hokkaido 0608589, Japan

来源：

TRENDS IN GLYCOSCIENCE AND GLYCOTECHNOLOGY | 2000年 / 12卷 / 68期

关键词：

alpha-glucosidase; intermediate; reaction mechanism; structural analysis of catalytic site; substrate recognition;

D O I：

10.4052/tigg.12.373

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

alpha -Glucosidase contributing search and glycogen metabolisms in plant and animal tissue is characterized by the variety in substrate recognition. Recent studies showed that a-glucosidases could be divided into two groups, family I and family II, in which family I enzymes belong to the cr-amylase family. This paper focusing on the difference in the a-glucosidase families reviews the structural information including the catalytic amino acid residues of nucleophile and acid/base catalyst, the recognition of substrate molecule, and the intermediate in the transition state of hydrolytic reaction.

引用

页码：373 / 380

页数：8

共 40 条

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STEREOSPECIFIC RING-OPENING OF CONDURITOL-B-EPOXIDE BY AN ACTIVE-SITE ASPARTATE RESIDUE OF SUCRASE-ISOMALTASE [J].