Fluorescence microscopical investigation of poly(3-hydroxybutyrate) granule formation in bacteria

被引：42

作者：

Jendrossek, D ^{[1
]}

机构：

[1] Univ Stuttgart, Inst Mikrobiol, D-70550 Stuttgart, Germany

来源：

BIOMACROMOLECULES | 2005年 / 6卷 / 02期

关键词：

D O I：

10.1021/bm049441r

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The early stages of poly(3-hydroxybutyrate) (PHB) accumulation were analyzed in vivo by fluorescence microscopy in Rhodospirillum rubrum, Ralstonia eutropha, and in recombinant Escherichia coli harboring the PHB biosynthesis genes phaCAB of R. eutropha. PHB granules were stained with Nile red and by expression of a phasin-enhanced yellow fluorescent protein fusion protein. Distribution of PHB granules at the early stages of PHB accumulation frequently occurred near the cell poles and near the cell wall in all three strains investigated. This is the first evidence obtained from living cells that PHB synthesis initiates not randomly but at discrete regions in bacteria.

引用

页码：598 / 603

页数：6

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