Advanced Technologies for Pathogen and Toxin Detection in Foods: Current Applications and Future Directions

被引:52
作者
Ge, Beilei [2 ]
Meng, Jianghong [1 ]
机构
[1] Univ Maryland, Dept Nutr & Food Sci, College Pk, MD 20742 USA
[2] Louisiana State Univ, Ctr Agr, Baton Rouge, LA 70803 USA
来源
JALA | 2009年 / 14卷 / 04期
关键词
detection; pathogen; toxin; food; advanced technology; MEDIATED ISOTHERMAL AMPLIFICATION; ESCHERICHIA-COLI O157-H7; REAL-TIME PCR; ETHIDIUM MONOAZIDE; LISTERIA-MONOCYTOGENES; ENVIRONMENTAL-SAMPLES; VIBRIO-VULNIFICUS; DEAD CELLS; IDENTIFICATION; ASSAY;
D O I
10.1016/j.jala.2008.12.012
中图分类号
Q5 [生物化学];
学科分类号
070307 [化学生物学];
摘要
Despite great strides made in the past decades, the detection of microbial pathogens and their toxins in foods remains a challenging task. This is due primarily to several inherent difficulties associated with food analysis, that is, the complexities of food matrices (inhibitors and normal flora), the attributes of target analytes in foods (low level, heterogeneous distribution, and cell injury during processing), and the ratio between the amount of food samples and the detection assay volume. This review aims to provide an overview and a better understanding of the limitations, current applications, and future perspectives in terms of pathogen and toxin detection in foods. (JALA 2009;14:235-241)
引用
收藏
页码:235 / 241
页数:7
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