Antiviral effect of strictinin on influenza virus replication

被引:62
作者
Saha, Repon Kumer [1 ,2 ]
Takahashi, Tadanobu [1 ,2 ]
Kurebayashi, Yuuki [1 ,2 ]
Fukushima, Keijo [1 ,2 ]
Minami, Akira [1 ,2 ]
Kinbara, Noriaki [3 ]
Ichitani, Masaki [3 ]
Sagesaka, Yuko M. [3 ]
Suzuki, Takashi [1 ,2 ]
机构
[1] Univ Shizuoka, Dept Biochem, Sch Pharmaceut Sci, Shizuoka 4228526, Japan
[2] Global COE Program Innovat Human Hlth Sci, Shizuoka 4228526, Japan
[3] Ito En Ltd, Ctr Res Inst, Makinohara 4210516, Japan
关键词
Strictinin; Virus; Influenza; Parainfluenza; Antiviral; Viral entry; GREEN TEA; PARAINFLUENZA VIRUS; IN-VITRO; A VIRUS; INHIBITION; TANNINS; IDENTIFICATION; NEURAMINIDASE; INFECTIVITY; SIALIDASE;
D O I
10.1016/j.antiviral.2010.06.008
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Strictinin, which is a member of the ellagitanin family of hydrolyzable tannins, prevented replication of human, duck and swine influenza A viruses (IAVs) in vitro at non-toxic concentrations. The addition of strictinin at the same time as IAV inoculation to MDCK cells inhibited viral replication in a dose-dependent manner. Strictinin showed 50% inhibitory concentrations for IAVs from 0.09 +/- 0.021 to 0.28 +/- 0.037 mu M (mean +/- S.E.M.) by the focus-forming assay. Treatment of MDCK cells with strictinin before and after viral inoculation resulted in no significant antiviral activity. Further studies showed that strictinin inhibited IAV-induced hemifusion. However, strictinin exhibited no inhibitory effect against receptor binding, sialidase activity. Strictinin also showed an antiviral effect on influenza B virus and human parainfluenza virus type-1 in vitro. The results indicate that strictinin is a useful antiviral agent. (C) 2010 Elsevier B.V. All rights reserved.
引用
收藏
页码:10 / 18
页数:9
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