Differential susceptibility to human immunodeficiency virus type 1 infection of myeloid and plasmacytoid dendritic cells

被引:173
作者
Smed-Sörensen, A
Loré, K
Vasudevan, J
Louder, MK
Andersson, J
Mascola, JR
Spetz, AL
Koup, RA
机构
[1] Karolinska Inst, Ctr Infect Med, Dept Med, S-14186 Stockholm, Sweden
[2] NIAID, Vaccine Res Ctr, Immunol Lab, NIH, Bethesda, MD 20892 USA
[3] NIAID, Vaccine Res Ctr, BSL 3 Virol Lab, NIH, Bethesda, MD 20892 USA
关键词
D O I
10.1128/JVI.79.14.8861-8869.2005
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Human immunodeficiency virus type 1 (HIV-1) infection of dendritic cells (DCs) plays an important role in HIV-1 transmission and pathogenesis. Here, we studied the susceptibility of ex vivo-isolated CD11c(+) myeloid DCs (MDCs) and CD123(+) plasmacytoid DCs (PDCs) to HIV-1 infection and the function of these cells early after infection. Both DC subsets were susceptible to CCR5- and CXCR4-using HIV-1 isolates (BaL and IIIB, respectively). However, MDCs were more susceptible to HIV-1(BaL) infection than donor-matched PDCs. In addition, HIV-1(BaL) infected MDCs more efficiently than HIV-1(IIIB), whereas PDCs were equally susceptible to both isolates. While exposure to HIV-1 alone resulted in only weak maturation of DCs, Toll-like receptor 7/8 ligation induced full maturation in both infected and uninfected DCs. Maturation did not increase HIV-1 replication in infected DO, and infected DCs retained their ability to produce tumor necrosis factor alpha after stimulation. Both HIV-1 isolates induced alpha interferon production exclusively in PDCs, irrespective of productive infection. In conclusion, PDCs and MDCs were susceptible to HIV-1 infection, but neither displayed functional defects as a consequence of infection. The difference in susceptibility of PDCs and MDCs to HIV-1 may have implications for HIV-1 transmission and DC-mediated transfer of HIV-1 to T cells.
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页码:8861 / 8869
页数:9
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