Phosphate stimulates differentiation and mineralization of the chondroprogenitor clone ATDC5

被引：24

作者：

Fujita, T ^{[1
]}

Meguro, T ^{[1
]}

Izumo, N ^{[1
]}

Yasutomi, C ^{[1
]}

Fukuyama, R ^{[1
]}

Nakamuta, H ^{[1
]}

Koida, M ^{[1
]}

机构：

[1] Setsunan Univ, Fac Pharmaceut Sci, Dept Pharmacol, Hirakata, Osaka 5730101, Japan

来源：

JAPANESE JOURNAL OF PHARMACOLOGY | 2001年 / 85卷 / 03期

关键词：

phosphate; ATDC5; endochondral osteogenesis;

D O I：

10.1254/jjp.85.278

中图分类号：

R9 [药学];

学科分类号：

1007 [药学];

摘要：

ATDC5 cells were employed to examine how inorganic phosphate (Pi) influences chondrocytic bone formation. 1) Pi (3 - 30 mM) plus ascorbic acid (50 mug/ml) dose-dependently accelerated proliferative differentiation and mineralization of ATDC5. 2) Northern blot analysis revealed that 10 mM Pi suppressed expression of type II collagen and PTH (parathyroid hormone) / PTH-related peptide (PTHrP) receptor, while it accelerated type X collagen expression. 3) Pi (3 - 30 mM) dose-dependently increased luciferase activity in the cells transfected with 3000 bp type X collagen promoter fused to the luciferase gene. The results suggest a regulatory role of Pi in endochondral osteogenesis.

引用

页码：278 / 281

页数：4

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