CREB;
human chondrocytes;
beta-endorphin;
mu-opioid receptor;
D O I:
10.1016/j.bbrc.2003.09.191
中图分类号:
Q5 [生物化学];
Q7 [分子生物学];
学科分类号:
071010 ;
081704 ;
摘要:
There is evidence of effects of morphine on cell proliferation and intraarticular morphine produces analgesia and has an anti-inflammatory effect in chronic arthritis. The effects of opioids are mediated through the G-protein-coupled receptors affecting the cAMP pathway. We demonstrated that human osteoarthritic cartilage and cultured chondrocytes possess the beta-opioid receptor. The presence of the receptor was shown by immunodetection, polymerase chain reaction, and Western blotting. Stimulation of chondrocytes with beta-endorphin resulted in decreased phosphorylation of the transcription factor cAMP responsive element binding protein (CREB). The effect was reversed by naltrexone. The obtained results indicate that in human articular chondrocytes opioids affect, via the mu-opioid receptor, the transcription factor CREB which in turn can cause subsequent changes in gene expression. (C) 2003 Elsevier Inc. All rights reserved.
机构:
SmithKline Beecham Pharmaceut, Dept Renal Pharmacol, King Of Prussia, PA 19406 USASmithKline Beecham Pharmaceut, Dept Renal Pharmacol, King Of Prussia, PA 19406 USA
Fitzgerald, LR
;
Mannan, IJ
论文数: 0引用数: 0
h-index: 0
机构:
SmithKline Beecham Pharmaceut, Dept Renal Pharmacol, King Of Prussia, PA 19406 USASmithKline Beecham Pharmaceut, Dept Renal Pharmacol, King Of Prussia, PA 19406 USA
Mannan, IJ
;
Dytko, GM
论文数: 0引用数: 0
h-index: 0
机构:
SmithKline Beecham Pharmaceut, Dept Renal Pharmacol, King Of Prussia, PA 19406 USASmithKline Beecham Pharmaceut, Dept Renal Pharmacol, King Of Prussia, PA 19406 USA
Dytko, GM
;
Wu, HL
论文数: 0引用数: 0
h-index: 0
机构:
SmithKline Beecham Pharmaceut, Dept Renal Pharmacol, King Of Prussia, PA 19406 USASmithKline Beecham Pharmaceut, Dept Renal Pharmacol, King Of Prussia, PA 19406 USA
Wu, HL
;
Nambi, P
论文数: 0引用数: 0
h-index: 0
机构:
SmithKline Beecham Pharmaceut, Dept Renal Pharmacol, King Of Prussia, PA 19406 USASmithKline Beecham Pharmaceut, Dept Renal Pharmacol, King Of Prussia, PA 19406 USA
机构:
SmithKline Beecham Pharmaceut, Dept Renal Pharmacol, King Of Prussia, PA 19406 USASmithKline Beecham Pharmaceut, Dept Renal Pharmacol, King Of Prussia, PA 19406 USA
Fitzgerald, LR
;
Mannan, IJ
论文数: 0引用数: 0
h-index: 0
机构:
SmithKline Beecham Pharmaceut, Dept Renal Pharmacol, King Of Prussia, PA 19406 USASmithKline Beecham Pharmaceut, Dept Renal Pharmacol, King Of Prussia, PA 19406 USA
Mannan, IJ
;
Dytko, GM
论文数: 0引用数: 0
h-index: 0
机构:
SmithKline Beecham Pharmaceut, Dept Renal Pharmacol, King Of Prussia, PA 19406 USASmithKline Beecham Pharmaceut, Dept Renal Pharmacol, King Of Prussia, PA 19406 USA
Dytko, GM
;
Wu, HL
论文数: 0引用数: 0
h-index: 0
机构:
SmithKline Beecham Pharmaceut, Dept Renal Pharmacol, King Of Prussia, PA 19406 USASmithKline Beecham Pharmaceut, Dept Renal Pharmacol, King Of Prussia, PA 19406 USA
Wu, HL
;
Nambi, P
论文数: 0引用数: 0
h-index: 0
机构:
SmithKline Beecham Pharmaceut, Dept Renal Pharmacol, King Of Prussia, PA 19406 USASmithKline Beecham Pharmaceut, Dept Renal Pharmacol, King Of Prussia, PA 19406 USA